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一般說明
使用克隆环，可以从含有许多克隆的培养板中分离并挑选出转染细胞的单个菌落。分离的克隆与周围细胞隔离，可解离并传代，或者用50-100μL生长培养基脉冲，以便分析分泌物。无菌克隆环的一端涂有润滑脂，以便密封克隆环与培养板的接触面。随后，在克隆环内将细胞解离和悬浮，并作为纯菌落转移到新的容器中。
使用电子束照射将这些克隆环灭菌。 以合适的剂量水平灭菌后，玻璃克隆环将呈棕色。
使用电子束照射将这些克隆环灭菌。 以合适的剂量水平灭菌后，玻璃克隆环将呈棕色。
包裝
包装在60 mm培养皿中，其中一端涂有硅脂
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