推荐产品
生物源
bovine
形狀
lyophilized powder
純化經由
heat shock fractionation
來源
USA origin
技術
Southern blotting: suitable
blocking: suitable
cell culture | mammalian: suitable
雜質
HIV I and HIVII, HCV and HBsAg, tested negative
pH值
7
適合性
suitable for microbiology
UniProt登錄號
異物活動
DNase free
DNase, RNase, NICKase, protease, none detected
NICKase free
Protease free
RNase free
儲存溫度
2-8°C
基因資訊
bovine ... ALB(280717)
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一般說明
Native bovine serum albumin (BSA) is a 66 kDa protein, which belongs to the family of serum albumin. It consists of three domains (I, II and III) and is the major protein found in circulatory system. Native BSA is an α-helical, globular and non-glycosylated protein, with 17-disulfide bonds.
Acetylation of bovine serum albumin modifies various residues, most notably lysine (Lys), and also serine (Ser) and threonine (Thr) to lesser extents. MALDI-TOF mass spectrometry studies on acetylated BSA have indicated a MW value about 3000 Da higher compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. Acetylation of BSA also modifies parallel amino acid residues in any trace contaminating nucleases in the BSA, which inactivates that trace nuclease content. Thus acetylated BSA can be used in molecular biology applications where trace nuclease activity must be minimized.
Acetylation of bovine serum albumin modifies various residues, most notably lysine (Lys), and also serine (Ser) and threonine (Thr) to lesser extents. MALDI-TOF mass spectrometry studies on acetylated BSA have indicated a MW value about 3000 Da higher compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. Acetylation of BSA also modifies parallel amino acid residues in any trace contaminating nucleases in the BSA, which inactivates that trace nuclease content. Thus acetylated BSA can be used in molecular biology applications where trace nuclease activity must be minimized.
應用
Acetylated bovine serum albumin has been used:
- as a component of protease buffer for protease inhibition assay
- as a blocking agent in the preparation of cDNA glass array
- to select hybridoma antibodies sensitive to M-ficolin epitopes by immunofluorometric assay
This B2518 acetylated BSA product has been cited in various publications and protocols, such as the following:
* As an electrophoresis standard: Lindberg, T. et al., J. Pediatr. Gastroenterol. Nutr., 27(1), 30-36 (1998)
* In in situ end-labeling buffer for labeling of DNA fragments: Save, V. et al., ”Detecting and Quantifying Apoptosis in Tissue Sections”, in Apoptosis Methods and Protocols (H.J.M. Brady, ed.). Methods Mol. Biol., Vol. 282, pp. 67-84 (2004)
* In EMSA studies on nucleocytoplasmic shuttling: Ghosh, C.C. et al., “Analysis of Nucleocytoplasmic Shuttling of NF?B Proteins in Human Leukocytes”, in Membrane Trafficking (A. Vancura, ed.). Methods Mol. Biol., Vol. 457, pp. 279-292 (2008)
* In selection buffer for ribosome display studies: Larman, H.B. et al., Nat. Protoc., 9(1), 90-103 (2014)
* As an electrophoresis standard: Lindberg, T. et al., J. Pediatr. Gastroenterol. Nutr., 27(1), 30-36 (1998)
* In in situ end-labeling buffer for labeling of DNA fragments: Save, V. et al., ”Detecting and Quantifying Apoptosis in Tissue Sections”, in Apoptosis Methods and Protocols (H.J.M. Brady, ed.). Methods Mol. Biol., Vol. 282, pp. 67-84 (2004)
* In EMSA studies on nucleocytoplasmic shuttling: Ghosh, C.C. et al., “Analysis of Nucleocytoplasmic Shuttling of NF?B Proteins in Human Leukocytes”, in Membrane Trafficking (A. Vancura, ed.). Methods Mol. Biol., Vol. 457, pp. 279-292 (2008)
* In selection buffer for ribosome display studies: Larman, H.B. et al., Nat. Protoc., 9(1), 90-103 (2014)
乙酰化的目的在于灭活 BSA 中通常存在的核酸酶。乙酰化 BSA 不能用作蛋白质标准品，因为酪氨酸残基乙酰化使得 Lowry 分析和其他类似蛋白质检测法无法显色。
生化／生理作用
Native bovine serum albumin (BSA) acts as a transporter of drugs, hormones and fatty acids. It has calcium binding sites and regulates metal homeostasis.
特點和優勢
- Acetylated to inactivate trace nucleases
- DNase free / Exonuclease free
- Nickase free / Endonuclease free
- RNase free
- Protease free
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Identification of genes regulated by interleukin-1beta in human endometrial stromal cells
Rossi M, et al.
Reproduction (Cambridge, England), 130(5), 721-729 (2005)
S A Fiscus et al.
Methods in molecular medicine, 20, 209-216 (1999-01-01)
Semen is the major vehicle for the sexual transmission of HIV-1. The ability to isolate infectious HIV from the semen and to quantitate viral burden in the form of cell-free or cell-associated HIV-1 RNA in semen are important for epidemiologic
Anne Troldborg et al.
Journal of clinical immunology, 39(4), 421-429 (2019-05-03)
Ficolin-3 deficiency is caused by a mutation (+1637delC) in the FCN3 gene. It is a rare condition and has been associated with both infection and autoimmune disease including systemic lupus erythematosus (SLE). Here we investigated if ficolin-3 deficiency is more
Characteristics and biological variations of M-ficolin, a pattern recognition molecule, in plasma
Wittenborn T, et al.
Journal of Innate Immunity, 2(2), 167-180 (2010)
Julie Brogaard Larsen et al.
Thrombosis and haemostasis, 119(6), 952-961 (2019-04-16)
Activation of the complement system is part of the dysregulated immune response in sepsis. The mannose-binding lectin-associated serine proteases (MASP)-1 and -2 activate the lectin pathway of the complement system. Besides, these proteins can activate coagulation in vitro. However, the
实验方案
Preparation for biodegradable nanoparticles and their use in transfection protocols .
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