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形狀
PBS suspension
品質等級
標籤範圍
≥30 mg avidin per mL packed resin
2-6 μmol per mL
基質
4% beaded agarose
基質活化
epoxy
基質結合
carboxy (amide linkage); resin remains relatively uncharged over wide pH range
基質墊片
13 atoms
應用
life science and biopharma
儲存溫度
2-8°C
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應用
生物素含有与抗生物素蛋白或链霉亲和素结合的高亲和力。它在生物化学和分子生物学中已被广泛用于检测和标记蛋白质。
外觀
混悬剂为含有0.02％叠氮化钠的磷酸盐缓冲盐水
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
K J Airenne et al.
Gene, 144(1), 75-80 (1994-06-24)
A recombinant avidin (re-Avd), containing amino acids (aa) 1-123 of the native chicken egg-white Avd, was produced in Escherichia coli. When cells were grown at 37 degrees C production was over 1 microgram/ml, due to altering the codon preference of
A P Sibler et al.
Journal of immunological methods, 224(1-2), 129-140 (1999-06-05)
In vivo biotinylation of antibody fragments with a gene fusion approach is a realistic alternative to conventional in vitro chemical labelling. We have previously reported the construction of a vector system suitable for the bacterial expression of the binding fragment
Emrah Celik et al.
Journal of molecular recognition : JMR, 25(1), 53-56 (2012-01-04)
Sample-probe contact duration (dwell time) and loading force are two important parameters for the atomic force microscopy (AFM) force spectroscopy measurements of ligand-receptor interaction. A prolonged contact time may be required to initiate ligand-receptor binding as a result of slow
P A Smith et al.
Nucleic acids research, 26(6), 1414-1420 (1998-04-29)
The high affinity binding interaction of biotin to avidin or streptavidin has been used widely in biochemistry and molecular biology, often in sensitive protein detection or protein capture applications. However, in vitro chemical techniques for protein biotinylation are not always
Biotinylated CdSe/ZnSe nanocrystals for specific fluorescent labeling.
Charvet, N., et al.
Journal of Materials Chemistry, 14, 2638-2642 (2004)
相关内容
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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