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A9543

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

heat shock fraction, low endotoxin, pH 7, ≥98%

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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About This Item

CAS号:
9048-46-8
EC號碼:
232-936-2
MDL號碼:
MFCD00130384
分類程式碼代碼:
12352202
NACRES:
NA.27

生物源

bovine

化驗

≥98%

形狀

lyophilized powder

分子量

~66 kDa

純化經由

heat shock fractionation

包裝

poly bottle of

來源

USA origin

技術

ELISA: suitable
electrophoresis: suitable
western blot: suitable

雜質

≤1.0 EU/mg endotoxin

損耗

≤5%

pH值

7

溶解度

water: soluble (40 mg/ml)

UniProt登錄號

P02769

異物活動

BT virus, none detected
VSV virus, none detected

儲存溫度

2-8°C

基因資訊

bovine ... ALB(280717)

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應用

This A9543 BSA product has been used in various systems, including:
  • NRK-52E rat kidney epithelioid cells: Ochiai, H. et al., J. Virol., 67(11), 6811-6814 (1993)
  • Neonatal porcine pancreatic islets: Ellis, C.E. et al., Islets, 5(5), 216-225 (2013)
  • Mouse:
Lee, D. et al., Am. J. Physiol. Renal Physiol., 304(7), F1009-F1019 (2013)
Girbl, T. et al., Immunity, 49(6), 1062-1076.e6 (2018)
  • iPSC cell line: Martin, H.C. et al., Science, 362(6419), 1161-1164 (2018).

生化／生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

特點和優勢

  • Heat shock fractionated
  • Low endotoxin

準備報告

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
Prepared using heat shock fractionation

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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