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生物源
bacterial (Bacillus amyloliquefaciens)
品質等級
種類
Type II-A
化驗
≥30%
形狀
lyophilized powder
比活性
≥1,500 units/mg protein (biuret)
分子量
50-55 kDa by SDS-PAGE
技術
SDS-PAGE: suitable
溶解度
H2O: soluble 0.1 mg/mL, clear, colorless
適合性
suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification
應用
life science and biopharma
儲存溫度
−20°C
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一般說明
αα-淀粉酶是一种胞外酶，广泛存在于许多动植物中，也分布于微生物中，例如各种芽孢杆菌(Bacillus species)。
α-淀粉酶（1,4-α-d-葡萄糖水解酶），是一种内切葡聚糖酶，属于糖苷水解酶家族13(GH13)的一员。
應用
α-淀粉酶(芽孢杆菌)已用于：
- 作为唾液组分，用于人工咀嚼；胃肠腔消化实验
- 作为十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)的标准品，测定α-淀粉酶浓度
- 对乙醇不溶物(AIR)脱淀粉，进行非纤维素神经单糖分析
生化／生理作用
α-淀粉酶可将淀粉降解为麦芽糖等寡糖、葡萄糖和α-极限糊精。它可水解由三个或多个α-(1→4)连接D-葡萄糖单元组成的多糖的α-(1→4)葡聚糖键，无需水解α-(1→6)键。参与葡萄糖生成，对于能量获取至关重要。α-淀粉酶是一种广为人知的工业酶，适用于食品、洗涤、纺织、发酵和制药行业。
特點和優勢
85 °C，pH 6.2下处理60分钟，依然保持>98%活性。
- α-淀粉酶（来自地衣芽孢杆菌NCIB 6346）在pH 6.2和85°C条件下60分钟后保留98%以上的活性。
- 其他α-淀粉酶在91°C下存放1小时后，仍然具有100%活性。
單位定義
在pH 6.9、温度20 °C条件下，一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。
準備報告
4×结晶
以0.1 mg/mL的浓度溶于水，形成透明无色溶液。
其他說明
本产品仅供研&发使用，不得用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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实验方案
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
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