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生物源
Porcine pancreas
品質等級
種類
Type I-A
形狀
saline suspension
比活性
700-1400 units/mg protein (E1%/280)
分子量
51-54 kDa
環保替代產品特色
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
技術
activity assay: suitable
適合性
suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification
應用
diagnostic assay manufacturing
環保替代類別
, Enabling
儲存溫度
2-8°C
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相关类别
一般說明
分子质量：51-54 kDa。
从猪胰腺中分离的α-淀粉酶是一种糖蛋白。它是由约475个残基组成的一条多肽链（包含2个SH基团和4个二硫键）以及一个紧密结合的Ca2+ （稳定性所必需的）组成的。氯离子是活性和稳定性所必需的。活性pH范围为5.5至8.0，最适pH为7。
从猪胰腺中分离的α-淀粉酶是一种糖蛋白。它是由约475个残基组成的一条多肽链（包含2个SH基团和4个二硫键）以及一个紧密结合的Ca2+ （稳定性所必需的）组成的。氯离子是活性和稳定性所必需的。活性pH范围为5.5至8.0，最适pH为7。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。当用于淀粉乙醇研究时，该产品经优化可提高能效和防止浪费。有关详细信息，请参阅《Biofiles》的文章。
應用
α-淀粉酶用于水解α-连接的多糖α键，如淀粉和糖原。Sigma 公司生产的α-淀粉酶已被用于各种植物研究，如拟南芥代谢研究。
生化／生理作用
α-淀粉酶可水解三个或更多 α-(1,4)连接的 D-葡萄糖单位多糖中的 α-(1,4)葡聚糖键。天然底物，如淀粉和糖原，被分解成葡萄糖和麦芽糖。来自猪胰腺的α-淀粉酶是一种糖蛋白，由约 475 个残基的单一多肽链组成，含有 2 个 SH 基团和 4 个二硫键以及保持稳定性所必需的紧密结合的 Ca2+。
單位定義
在pH 6.9、温度20 °C条件下，一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。
外觀
悬浮于含 3 mM CaCl2 的 2.9 M NaCl 溶液中。
準備報告
2 次结晶
其他說明
有关复合碳水化合物分析 酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Diabetes is a common disease caused by a combination of genetic and environmental factors, which was the top three diseases threatening human health. Therefore, it is necessary to seek more efficient hypoglycemic drugs. The main objective of this study was
Annabel Bijttebier et al.
Carbohydrate research, 345(2), 235-242 (2009-12-08)
Amylopectin fine structures were studied following limited hydrolysis of gelatinised waxy maize starch by amylases with a different level of inner chain attack (LICA). This was done by size exclusion chromatography as well as by debranching the (partially hydrolysed) amylopectin
Saber Abdelkader Saidi et al.
Heliyon, 8(12), e11954-e11954 (2022-12-09)
The study evaluated the phytochemical composition of Ephedra alata and its effects on α-amylase and lipase enzymes and diabetic-induced liver-kidney-testes toxicities to determine the anti-diabetic, anti-obesity, and anti-toxic potentials of the plant. Obesity was induced by a high-fat and fructose
Buford L Nichols et al.
The Journal of nutrition, 139(4), 684-690 (2009-02-06)
Starch is the major source of food glucose and its digestion requires small intestinal alpha-glucosidic activities provided by the 2 soluble amylases and 4 enzymes bound to the mucosal surface of enterocytes. Two of these mucosal activities are associated with
Ana M Rovalino-Córdova et al.
Carbohydrate polymers, 253, 117351-117351 (2020-12-07)
The role of the plant matrix is recognized as the main factor restricting starch digestibility in beans. Several authors have provided insights about the mechanisms behind the reduced starch digestibility in plant matrices. In this study, by means of a
实验方案
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
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