推荐产品
生物源
bovine
品質等級
化驗
≥96%
形狀
lyophilized powder
分子量
~66 kDa
純化經由
heat shock fractionation
包裝
poly bottle of
來源
USA origin
技術
blood typing: suitable
microbiological culture: suitable
損耗
≤5%
pH值
5.2
溶解度
water: soluble (40 mg/ml)
UniProt登錄號
異物活動
BT Virus, none detected
VSV Virus, none detected
儲存溫度
2-8°C
基因資訊
bovine ... ALB(280717)
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一般說明
Bovine serum albumin (BSA) is a 66.4 kDa, water-soluble protein composed of 583 amino acids. It has a single polypeptide chain with three homologous domains formed by six α-helices. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating. BSA has been used as a model protein in various applications, including immunodiagnostic procedures, cell culture media and clinical chemistry. Serum albumins facilitates drug disposition and efficacy by maintaining osmotic blood pressure.
應用
Bovine Serum Albumin (BSA) has been used:
- to study its adsorption on a planar poly(acrylic acid) (PAA) brush layer through fixed-angle optical reflectometry
- to measure its adsorption on a variety of thin films by means of total internal reflection spectroscopy
- as a constituent of Krebs-Ringer bicarbonate buffer, which acts as a perfusate during placental perfusion
生化／生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
品質
残余物主要是球蛋白。
準備報告
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Adsorption of the protein bovine serum albumin in a planar poly (acrylic acid) brush layer as measured by optical reflectometry
de Vos WM, et al.
Langmuir, 24(13), 6575-6584 (2008)
Functional role of P-glycoprotein in the human blood-placental barrier
Molsa M, et al.
Clinical Pharmacology and Therapeutics, 78(2), 123-131 (2005)
Real-time analysis of protein adsorption to a variety of thin films
Sapsford KE and Ligler FS
Biosensors And Bioelectronics, 19(9), 1045-1055 (2004)
Yiwen Sun et al.
Biomedical optics express, 9(7), 2917-2929 (2018-07-10)
The development of new spectral analysis methods in bio thin-film detection has generated intense interest in terahertz (THz) spectroscopy and its application in a wide range of fields. In this paper, it is the first time that machine learning methods
Study of the interaction between monoammonium glycyrrhizinate and bovine serum albumin.
Hu Y J, et al.
Journal of Pharmaceutical and Biomedical Analysis, 36(4), 915-919 (2004)
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