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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
2G11, monoclonal
形狀
buffered aqueous solution
分子量
antigen 60-80 kDa (glycosylated form)
物種活性
pig, mouse, rabbit, rat, bovine, ferret, canine
技術
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:2,000
western blot (chemiluminescent): 1:4,000
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
mouse ... Atp4b(11945)
rat ... Atp4b(24217)
一般說明
H+/K+-ATPase是胃质子泵，是P型阳离子转运腺苷5′-三磷酸酶（ATPase）家族的成员。H+/K+-ATPase由一个大的跨膜催化α亚基组成，其中包含ATP结合和磷酸化的位点。该ATP酶还具有较小的糖蛋白（β）亚基，其可能在维持复合物的结构和功能完整性中起作用。小鼠单克隆抗H+/K+ ATPase（β亚基）与牛，狗，猪，兔，小鼠，雪貂和大鼠组织中的H+/K+-ATPase β亚基结合。通过免疫印迹，该抗体可检测各种形式的β亚基，包括60-80 kDa糖基化蛋白，52 kDaβ亚基前体和34 kDa核心肽。
该抗体可与各种形式的H+/K+ TPaseβ亚基反应。它结合位于β亚基细胞质侧的氨基酸1-13或15-28内的表位。已显示该抗体抑制H+/K+-ATPase的酶活性并改变细胞质K+结合位点的亲和力。它可用于定位和检测H+/K+ TPaseβ亚基。
免疫原
来自去糖基化猪胃微粒体的核34 kDa H+/K+ ATPase β 亚基。
應用
小鼠单克隆抗H+/K+ ATPase（β亚基）抗体可用于间接免疫荧光（1:2,000）、化学发光免疫印迹（1:4,000）、免疫组化等应用。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质印迹（1篇论文）
蛋白质印迹（1篇论文）
外觀
含有 0.05% 叠氮化钠的磷酸盐缓冲盐水溶液。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Ivana Novak et al.
The Journal of biological chemistry, 286(1), 280-289 (2010-10-28)
Pancreas secretes fluid rich in digestive enzymes and bicarbonate. The alkaline secretion is important in buffering of acid chyme entering duodenum and for activation of enzymes. This secretion is formed in pancreatic ducts, and studies to date show that plasma
Salim M A Bastaki et al.
Molecular and cellular biochemistry, 341(1-2), 43-50 (2010-03-20)
Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats
D C Chow et al.
The American journal of physiology, 265(6 Pt 1), C1562-C1570 (1993-12-01)
The gastric proton pump, H(+)-K(+)-ATPase, is composed of alpha- and beta-subunits. The 95-kDa alpha-subunit has been referred to as the catalytic subunit containing sites for ATP binding and phosphorylation. The beta-subunit is a glycoprotein with a 34-kDa core peptide that
Marco Tozzi et al.
Cancers, 12(3) (2020-03-14)
Pancreatic duct cells are equipped with acid/base transporters important for exocrine secretion. Pancreatic ductal adenocarcinoma (PDAC) cells may utilize such transporters to acidify extracellular tumor microenvironment, creating a niche favoring cell proliferation, fibrosis and resistance to chemotherapy-all contributing to the
Jing Wang et al.
PloS one, 10(5), e0126432-e0126432 (2015-05-21)
The mechanism by which pancreas secretes high HCO3- has not been fully resolved. This alkaline secretion, formed in pancreatic ducts, can be achieved by transporting HCO3- from serosa to mucosa or by moving H+ in the opposite direction. The aim
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