A2105

Anti-AP Endonuclease antibody, Mouse monoclonal

clone APEREF, purified from hybridoma cell culture

• 别名: Anti-AP lysase, Anti-APE/Ref1, Anti-APE1, Anti-APEN, Anti-APEX nuclease, Anti-APEX1, Anti-Apurinic/Apyrimidinic Endonuclease, Anti-HAP1, Monoclonal Anti-AP Endonuclease antibody produced in mouse
• MDL號碼: MFCD01633033
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: APEREF, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~37 kDa
• 物種活性: canine, human, rat, mouse
• 濃度: ~2 mg/mL
• 技術: immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.5-1 μg/mL using total cell extract of Raji cells
• 同型: IgG1
• UniProt登錄號: P27695
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... APEX1(328) ; mouse ... Apex1(11792) ; rat ... Apex1(79116)

说明

一般說明

AP endonuclease (APE/Ref1 or Apurinic/apyrimidinic endonuclease) is a crucial enzyme in the DNA base excision repair (BER) pathway. It recognizes baseless sites in the DNA following spontaneous base loss or removal of a damaged base by different DNA glycosylases. This enzyme is the major AP endonuclease in mammalian cells. The human enzyme shows a high degree of homology with other mammalian AP endonucleases (bovine, mouse, rat, and hamster). APE/Ref1 is a multifunctional enzyme. Its N-terminal region includes a nuclear localization sequence and a redox activity zone, while its AP endonuclease activity resides in the C-terminal region. Redox-factor 1 (Ref1) refers to the reduction/oxidation function of the enzyme.

Monoclonal Anti-AP Endonuclease recognizes human, canine, rat, and mouse AP endonuclease (approx. 37kDa).

The AP endonuclease is a DNA damage repair gene involved in base excision repair.

免疫原

recombinant human AP endonuclease.

應用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal anti-AP endonuclease antibodies have been used for western blot analysis. The antibody can also be used for microarray, indirect ELISA and immunocytochemistry assays.

生化／生理作用

AP endonuclease (APE/Ref1 or Apurinic/apyrimidinic endonuclease) has a 3′-repair diesterase or phosphatase activity. The major DNA repair activity of the enzyme is nicking the DNA phosphodiester backbone 5′ to the AP site leaving a 3′-hydroxyl group and a 5′-deoxyribose phosphate. APE/Ref1 stimulates the DNA binding activity of many transcription factors (Fos, Jun, NF?B, PAX, HIF1, p53 and CREB), which are involved in cancer promotion and progression. The protein was found at elevated levels in several tumors (ovarian, prostate, cervical and germ cells). Redox-factor 1 (Ref1) maintains transcription factors in their active reduced states.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.