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應用
氨基烯丙基-dUTP可用于通过聚合酶链式反应(PCR),在第一链cDNA的合成过程中进行间接稳定标记。氨基烯丙基-dUTP可用作反应性位点,用于后续的反应性荧光探针的标记。氨基烯丙基-dUTP也可用于构建反应性DNA支架。
氨基烯丙基-dUTP钠盐已被用于:
- 添加至脱氧核苷三磷酸混合物中用于互补DNA(cDNA)的合成
- 生成染色质免疫沉淀(ChIP)探针
- 在RNA的逆转录中用于合成荧光标记的探针
生化/生理作用
氨基烯丙基-dUTP钠盐/5-(3-氨基烯丙基)-2′-脱氧尿苷5′-三磷酸(aa-dUTP)主要用于合成核酸杂交探针的两步标记法中。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes.
Appl. Immunohistochem., 15, 332-337 (2007)
BioTechniques, 36(1), 114-122 (2004-01-27)
Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP) is enzymatically incorporated to generate amine-modified DNA, which is
World journal of gastroenterology, 13(3), 341-348 (2007-01-19)
To identify tumor associated genes of rectal cancer and to probe the application possibility of gene expression profiles for the classification of tumors. Rectal cancer tissues and their paired normal mucosa were obtained from patients undergoing surgical resection of rectal
mBio, 4(2) (2013-03-28)
Light is a pervasive environmental factor that regulates development, stress resistance, and even virulence in numerous fungal species. Though much research has focused on signaling pathways in Aspergillus fumigatus, an understanding of how this pathogen responds to light is lacking.
Effect of the Colorless non-ripening mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening
Plant Physiology, 136(4), 4184-4197 (2004)
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