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等級
for fluorescence
化驗
≥99.0% (HPLC)
形狀
solid
雜質
≤0.5% phthalic acid
mp
54-56 °C
螢光
λex 334 nm; λem 455 nm (Thiol Adduct)
λex 340 nm; λem 450 nm in reaction buffer (with glycine)
儲存溫度
2-8°C
SMILES 字串
O=Cc1ccccc1C=O
InChI
1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H
InChI 密鑰
ZWLUXSQADUDCSB-UHFFFAOYSA-N
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一般說明
邻苯二甲醛,又称o-邻苯二甲醛或OPA 是一种柱前衍生化试剂。OPA与除胱氨酸外的主要氨基酸 (AA) 反应形成高荧光加合物。其衍生物的荧光激发光谱和发射光谱分别为430 nm和540 nm。HPLC技术可有效分离OPA-AA衍生物。
應用
邻苯二甲醛或OPA可测定动物组织和食品样品中蛋白质中的氨基酸。对乳酸菌产生的酪胺和苯乙胺的氨基酸加合物进行定量,使用OPA作为HPLC的柱前衍生试剂。使用邻苯二甲醛作为柱前荧光剂,研究细胞内谷胱甘肽(GSH/GSSG)的形式。
生化/生理作用
苯二醛可用于高效液相色谱(HPLC)分离的氨基酸柱前衍生化。还可用于蛋白质巯基的流式细胞术测定。
訊號詞
Danger
危險分類
Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
水污染物質分類(WGK)
WGK 3
閃點(°F)
269.6 °F - closed cup
閃點(°C)
132 °C - closed cup
個人防護裝備
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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The ability of wine lactic acid bacteria to produce tyramine and phenylethylamine was investigated by biochemical and genetic methods. An easy and accurate plate medium was developed to detect tyramine-producer strains, and a specific PCR assay that detects the presence
Food research international (Ottawa, Ont.), 145, 110430-110430 (2021-06-12)
Flavonoid bioavailability and bioactivity is associated with interindividual variability, which is partially due to differences in health status. Previously, it was demonstrated that cellular stress, especially mitochondrial stress, increases intracellular quercetin uptake and this is associated with beneficial health effects.
Applied microbiology and biotechnology, 99(16), 6715-6726 (2015-02-17)
Sphingolipid ceramide N-deacylase (SCDase) catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. While SCDases show great value for the enzymatic synthesis of glycosphingolipids, they are relatively poorly characterized enzymes. In this work, the enzymatic
Scandinavian journal of work, environment & health, 41(2), 124-139 (2015-01-20)
This study aimed to assess the effects of physically demanding work - measured as energy expenditure (EE) during occupational physical activities (OPA) - on risk of acute myocardial infarction (AMI) among men with and without preexisting ischemic heart disease (IHD).
The New phytologist, 193(1), 51-57 (2011-11-11)
• Sphingolipids are emerging as important mediators of cellular and developmental processes in plants, and advances in lipidomics have yielded a wealth of information on the composition of plant sphingolipidomes. Studies using Arabidopsis thaliana showed that the dihydroxy long-chain base
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