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Merck

70540

Sigma-Aldrich

萘酚黄 S

for microscopy (Hist.), for the precipitation (of amino acids and peptides)

别名:

2,4-二硝基-1-萘酚-7-磺酸 钠盐, 8-羟基-5,7-二硝基-2-萘磺酸 钠盐, 酸性黄 1, 黄胺酸 钠盐

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About This Item

经验公式(希尔记法):
C10H4N2Na2O8S
CAS号:
分子量:
358.19
顏色索引編號:
10316
Beilstein:
3839220
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47

等級

for microscopy (Hist.)
for the precipitation (of amino acids and peptides)

形狀

powder

溶解度

methanol: water (1:1): 0.5 g/10 mL, clear

εmax

≥275 at 423-433 nm in water
≥≥ 250 at 387-397 nm in water

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2

InChI 密鑰

CTIQLGJVGNGFEW-UHFFFAOYSA-L

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應用

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.

生化/生理作用

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.

分析報告

λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)

象形圖

Health hazardExclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1 - STOT RE 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
N W Schipper et al.
Laboratory investigation; a journal of technical methods and pathology, 61(2), 228-234 (1989-08-01)
The paper describes an improved segmentation method to measure the percentages of epithelium and stroma in ovarian (tumor) tissue with automated image analysis and evaluates its prognostic value. In the image processing method, a blue-yellow image pair is recorded from
Brodsky VYa et al.
European journal of histochemistry : EJH, 37(3), 199-206 (1993-01-01)
Two-wavelength scanning cytophotometry was performed for the measurement of DNA (Feulgen reaction) and total protein content (naphthol yellow S staining) in the same cardiac myocyte. A lack of proportionality between the DNA content (i.e, cell ploidy) and protein content has
Cell and Tissue Culture in Forestry (2012)
J Tas et al.
The Histochemical journal, 13(5), 711-716 (1981-09-01)
The 'total protein staining' of biological specimens with the electrostatically binding Naphthol Yellow S or the covalently binding dinitrofluorobenzene must be interpreted as methods which yield data on the specific amino acid pool of the proteins concerned. Both dyes bind

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