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Merck

45245

Sigma-Aldrich

曙红5-异硫氰酸酯

≥95.0% (UV)

别名:

EITC

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About This Item

经验公式(希尔记法):
C21H7Br4NO5S
CAS号:
分子量:
704.96
MDL號碼:
分類程式碼代碼:
12352200
PubChem物質ID:

化驗

≥95.0% (UV)

螢光

λex 521; λem 544 (pH 9.0)

儲存溫度

2-8°C

SMILES 字串

OC(=O)c1cc(ccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c(O)c(Br)cc24)N=C=S

InChI

1S/C21H7Br4NO5S/c22-12-4-10-14(8-2-1-7(26-6-32)3-9(8)21(29)30)11-5-13(23)18(28)16(25)20(11)31-19(10)15(24)17(12)27/h1-5,27H,(H,29,30)

InChI 密鑰

MCXYFYWNOWPDMA-UHFFFAOYSA-N

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應用

曙红5-异硫氰酸酯(EITC)是一种荧光探针。在荧光共振能量转移(FRET)测定中,EITC标记的分子可与荧光素-5′-异硫氰酸酯(FITC)标记的分子一起使用。

其他說明

受体荧光团。测定蛋白质的旋转扩散;用于荧光能量转移研究

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Ya-lan Wu et al.
Guang pu xue yu guang pu fen xi = Guang pu, 24(12), 1597-1600 (2005-04-15)
In this paper, the medium (pH, organic solvent and surfactant) effects on fluorescence of eosin-5-isothiocyanate (eosin-ITC) have been studied in detail. The results show that the pH and polarity of system studied are two great important influence factors on fluorescence
M Schindler et al.
The Journal of cell biology, 92(3), 742-746 (1982-03-01)
Chemical modification of amino groups in matrix porin solubilized and purified from outer membranes of Escherichia coli in beta-octylglucoside was performed with eosin isothiocyanate and citraconic anhydride. At pH 7 8.5, the former reagent labeled a single amino group in
R Wagner et al.
European journal of biochemistry, 173(3), 561-568 (1988-05-02)
Phosphoenolpyruvate carboxylase, purified from maize leaves, is rapidly inactivated by the fluorescence probe dansyl chloride. The loss of activity can be ascribed to the covalent modification of an R-NH2 group, presumably the epsilon-NH2 group of lysine. Analysis of the data
R Mittal et al.
Biochemistry, 33(33), 10178-10184 (1994-08-23)
We have used resonance energy transfer to read out the interactions of the alpha subunit of transducin (alpha T) with the transducin beta gamma subunit complex (beta gamma T) and to compare the rate of aluminum fluoride-induced alpha T activation
Yan Fu et al.
Journal of immunological methods, 289(1-2), 57-64 (2004-07-15)
A solid substrate room temperature phosphorescence immunoassay (SS-RTP-IA) for the determination of human complement3 (C3) based on a sandwich type assay and a labelled avidin-biotin (LAB) type assay was described. The anti-human complement3 and avidin were labeled with eosin5-isothiocyanate. On

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