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生物源
hamster ovary
描述
Hamster Chinese Ovary, serum-free
增長模式
suspension
形態學
Aggregates in suspension
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系描述
This is a derviative of the CHO-K1 cell line, ECACC catalogue no. 85051005 which has been adapted to grow in the animal component free medium, CHO PF Media (C5467)
培養基
CHO PF Media (C5467) + 8mM L-Glutamine (G7513). When freezing cells use freeze medium composed of 50:50 fresh:conditioned medium + 10% DMSO.
例行更新培養
Maintain cultures between approximately 3-9 x 105 cells/ml; 5% CO2; 37°C. Cells grow as aggregates in suspension. Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days.
其他說明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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