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Merck

10419

Sigma-Aldrich

8-苯胺基-1-萘磺酸 半镁盐 水合物

for fluorescence, ≥95.0% (T)

别名:

8-苯胺-1-萘磺酸镁 水合物, ANSA 镁盐, N-苯基-1-萘胺-8-磺酸 镁盐, 苯基-1-萘胺-8-磺酸 镁盐

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About This Item

经验公式(希尔记法):
C16H12Mg0.5NO3S · xH2O
CAS号:
分子量:
310.49 (anhydrous basis)
Beilstein:
4834623
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47

等級

for fluorescence

品質等級

化驗

≥95.0% (T)

形狀

powder

技術

titration: suitable

溶解度

hot water: 50 mg/mL, green

λmax

465-475 nm

螢光

λex 388 nm; λem 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

O.[Mg++].[O-]S(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12.[O-]S(=O)(=O)c4cccc5cccc(Nc6ccccc6)c45

InChI

1S/2C16H13NO3S.Mg.H2O/c2*18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13;;/h2*1-11,17H,(H,18,19,20);;1H2/q;;+2;/p-2

InChI 密鑰

FBANPIUXAXTAFX-UHFFFAOYSA-L

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一般說明

ANSA (8-Anilino-1-naphthalensulfonic acid) 是一种疏水性极性敏感的荧光染料,可作为位点探针用于检测细胞和胶束膜以及蛋白质等分子的构象变化。ANSA 也用作荧光探针,用于估算表面活性剂的临界胶束浓度 (CMC)。

特點和優勢

用于估算表面活性剂CMC的荧光探针。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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J L Jensen et al.
Cellular and molecular biology (Noisy-le-Grand, France), 46(3), 685-696 (2000-06-29)
We have studied the metal-catalyzed oxidation (MCO) of brain-derived neurotrophic factor (BDNF) with regard to target sites and potential conformational changes of the protein. The exposure of BDNF to three different levels of ascorbate/Cu(II)/O2 [20 microM Cu(II), 2 mM ascorbate
Rapid fluorescent "staining" of nondenatured protein bands in agar and polyacrylamide gels.
S T Nerenberg et al.
Analytical biochemistry, 43(2), 564-574 (1971-10-01)
C D Peters et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 75(1), 55-61 (1997-01-01)
Protein aggregation is believed to be due to conformers that expose hydrophobic clusters that promote protein association. Such conformers can be detected using a fluorescent probe like 8-anilino 1-naphthalenesulfonic acid (ANSA). Here we show that urease exposed to 1.0 M
A fluorimetric method for the estimation of the critical micelle concentration of surfactants.
E De Vendittis et al.
Analytical biochemistry, 115(2), 278-286 (1981-08-01)
F-O McDuff et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 82(2), 305-313 (2004-04-03)
Canavalia ensiformis (jack bean) alpha-urease is a hexameric protein characterized by a complex denaturation mechanism. In previous papers, we have shown that a hydrophobic 8-anilino-1-naphthalenesulfonic acid (ANSA) binding conformer could be populated in a moderate concentration of denaturant. This state

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