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Merck

02744

Sigma-Aldrich

Atto 655-Streptavidin

BioReagent, suitable for fluorescence

别名:

Streptavidin-Atto 655

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

產品線

BioReagent

品質等級

形狀

powder

製造商/商標名

ATTO-TEC GmbH

溶解度

DMF: soluble
DMSO: soluble
acetonitrile: soluble

螢光

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

適合性

suitable for fluorescence
suitable for gel electrophoresis

儲存溫度

−20°C

應用

Atto 655 is a fluorescent label with high molecular absorption (125.000) and quantum yield (0.30), as well as sufficient stoke′s shift (excitation maximum 663 nm, emission maximum 684 nm). There is very little cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Atto 655 has been coupled to streptavidin to enable various types of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays.

分析報告

free of unconjugated dye

法律資訊

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Simple buffers for 3D STORM microscopy.
van de Linde, S.; Kasper, R.; Heilemann, M.; Sauer, M.
Applied Physics B: Lasers and Optics, 93(4), 725-731 (2008)
Triple-Color Super-Resolution Imaging of Live Cells. Resolving Submicroscopic Receptor Organization in the Plasma Membrane.
Wilmes, S.; Staufenbiel, M.; Lisse, D.; Richter, Ch. P.; Beutel, O.; Busch, K. B. et al.
Angewandte Chemie (International Edition in English), 124(20), 4952-4955 (2012)
Nicolas Olivier et al.
Biomedical optics express, 4(6), 885-899 (2013-06-14)
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging:
PALM and STORM. Unlocking live-cell super-resolution.
Henriques, R.; Griffiths, C.; Hesper Rego, E.; Mhlanga, Musa M.
Biopolymers, 95(5), 322-331 (2011)

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Fluorescent Labeling of Peptides

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