跳转至内容
Merck

E7889

Sigma-Aldrich

乙二胺四乙酸 二钠盐 溶液

for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

别名:

EDTA 二钠盐, EDTA-Na2, 亚乙基二次氮基四乙酸 二钠盐

登录查看公司和协议定价


About This Item

线性分子式:
[-CH2N(CH2CO2Na)CH2CO2H]2
CAS号:
分子量:
336.21
Beilstein:
3822669
MDL號碼:
分類程式碼代碼:
12352107
PubChem物質ID:
NACRES:
NA.31

等級

for molecular biology

品質等級

無菌

0.2 μm filtered

形狀

liquid

反應適用性

reagent type: chelator

濃度

0.5 M in H2O

pH值

7.5-8.5

適合性

suitable for molecular biology

應用

microbiology

異物活動

DNase, RNase, NICKase and protease, none detected

SMILES 字串

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

InChI 密鑰

ZGTMUACCHSMWAC-UHFFFAOYSA-L

正在寻找类似产品? 访问 产品对比指南

一般說明

乙二胺四乙酸二钠盐溶液是一种螯合剂,适用于分子生物学应用。
乙二胺四乙酸(EDTA)是一种多元酸。EDTA中带孤对电子的四个羧酸和两个胺基可以螯合钙离子及其他几种金属离子。

應用

乙二胺四乙酸二钠盐溶液已应用于:
  • 盐酸(HCL)中消化蛋白酶-K,以测定角膜样本中DNA的含量
  • 与木瓜蛋白酶结合消化角膜样本,以量化糖胺聚糖(GAG)的含量
  • 预处理中,以剥离生长至80%融合的上皮细胞
  • 制备裂解缓冲液的原液
  • 制备尿素裂解缓冲液

特點和優勢

分子生物学级

準備報告

用分子生物学试剂 EDTA (E 5134) 在 18 兆欧水中制备

其他說明

如需了解生化试剂系列的更多信息,请填写此表

象形圖

Health hazard

訊號詞

Warning

危險聲明

防範說明

危險分類

STOT RE 2 Inhalation

標靶器官

Respiratory Tract

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Marta Filipiak et al.
Fish physiology and biochemistry, 38(2), 355-362 (2011-05-27)
The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken
Jarrod S Johnson et al.
Cell host & microbe, 23(3), 366-381 (2018-03-16)
Myeloid dendritic cells (DCs) have the innate capacity to sense pathogens and orchestrate immune responses. However, DCs do not mount efficient immune responses to HIV-1, primarily due to restriction of virus reverse transcription, which prevents accumulation of viral cDNA and
Yongchao Dou et al.
Cell, 180(4), 729-748 (2020-02-16)
We undertook a comprehensive proteogenomic characterization of 95 prospectively collected endometrial carcinomas, comprising 83 endometrioid and 12 serous tumors. This analysis revealed possible new consequences of perturbations to the p53 and Wnt/β-catenin pathways, identified a potential role for circRNAs in
Ci Chu et al.
Molecular cell, 44(4), 667-678 (2011-10-04)
Long noncoding RNAs (lncRNAs) are key regulators of chromatin state, yet the nature and sites of RNA-chromatin interaction are mostly unknown. Here we introduce Chromatin Isolation by RNA Purification (ChIRP), where tiling oligonucleotides retrieve specific lncRNAs with bound protein and
Joshua B Black et al.
Cell reports, 33(9), 108460-108460 (2020-12-03)
Technologies to reprogram cell-type specification have revolutionized the fields of regenerative medicine and disease modeling. Currently, the selection of fate-determining factors for cell reprogramming applications is typically a laborious and low-throughput process. Therefore, we use high-throughput pooled CRISPR activation (CRISPRa)

实验方案

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门