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Merck

C2734

Sigma-Aldrich

5-羧基四甲基罗丹明

≥95% purity (HPLC), solid

别名:

5-TAMRA

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About This Item

经验公式(希尔记法):
C25H22N2O5
CAS号:
分子量:
430.45
MDL號碼:
分類程式碼代碼:
12171500
NACRES:
NA.47

product name

5-羧基四甲基罗丹明,

化驗

≥95% (HPLC)

品質等級

形狀

solid

溶解度

water: slightly soluble

&epsilon ;(消光係數)

≥85000-97000 at 539-545 nm in methanol

螢光

λex ~543 nm; λem ~570 nm in methanol

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

InChI 密鑰

YMZMTOFQCVHHFB-UHFFFAOYSA-N

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一般說明

在高纯度中制备的重要荧光基团,通过约 600 个分子的毛细管电泳检测限为 TMR 标记氨基酸。容易被荧光显微镜中使用的汞弧灯的 546 nm 光谱线激发,本质上比荧光素更具光稳定性。

應用

5-羧基四甲基罗丹明已用于荧光团表征。

生化/生理作用

5-羧基四甲基罗丹明可以共价连接到 DNA 上,从而用于涉及分支和双链 DNA/RNA 结构的研究。主要用于单分子研究。5-羧基四甲基罗丹明也用于标记寡糖,用于激光诱导荧光检测。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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G Vámosi et al.
Biophysical journal, 71(2), 972-994 (1996-08-01)
Fluorescence steady-state and lifetime experiments have been carried out on duplex and single-stranded DNA molecules labeled at the 5' ends with 5-carboxytetramethylrhodamine (TMRh). The temperature and ionic strength of the solutions were varied over large ranges. The results reveal at
Magali Court et al.
Molecular & cellular proteomics : MCP, 16(12), 2153-2168 (2017-09-10)
Macrophages are innate immune cells which can react to a large number of environmental stimuli thanks to a high degree of plasticity. These cells are involved in a variety of tissue functions in homeostasis, and they play essential roles in
Limary M Cancel et al.
Scientific reports, 8(1), 9265-9265 (2018-06-20)
We investigated the effects of direct current stimulation (DCS) on fluid and solute transport across endothelial cell (EC) monolayers in vitro. Our motivation was transcranial direct current stimulation (tDCS) that has been investigated for treatment of neuropsychiatric disorders, to enhance
Giovanni Luongo et al.
ACS applied materials & interfaces, 9(46), 40059-40069 (2017-10-13)
Iron oxide nanostructures have been widely developed for biomedical applications because of their magnetic properties and biocompatibility. In clinical applications, stabilization of these nanostructures against aggregation and nonspecific interactions is typically achieved using weakly anchored polysaccharides, with better-defined and more
Xinyi Chen et al.
PloS one, 8(12), e80390-e80390 (2013-12-07)
Previous research suggests that carbohydrate mimetic peptide IF7 (IFLLWQR) has an excellent targeting property to annexin1 (Anxa1), a specific marker on the tumor endothelium. However, IF7 is susceptible to proteolysis and has a poor stability in vivo. We prepared a

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