21819

按照 Kinyoun 的石碳酸复红溶液

for microscopy

• 别名: Kinyoun 石碳酸复红
• MDL號碼: MFCD00143924
• 分類程式碼代碼: 12171500
• PubChem物質ID: 329752209
• NACRES: MA.02

属性

• 等級: for microscopy
• 品質等級: 100
• 形狀: liquid
• 成份: fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)
• 雜質: phenol
• 顏色: red to very dark red
• 密度: 0.99 g/mL at 20 °C
• &epsilon ；(消光係數): 77 at 546 nm in 50% ethanol
• 應用: diagnostic assay manufacturing; hematology; histology
• 儲存溫度: room temp
• SMILES 字串: NC1=CC=C(C(C2=CC=C(N)C=C2)=C(C=C3)C=CC3=[NH2+])C=C1.[Cl-]
• InChI: 1S/C21H21N3/c1-13-11-16(5-9-19(13)23)21(15-3-7-18(22)8-4-15)17-6-10-20(24)14(2)12-17/h3-12,22H,23-24H2,1-2H3
• InChI 密鑰: IMOKRVRFBCWWGH-UHFFFAOYSA-N

说明

一般說明

Carbol-Fuchsin is an acid-fast stain that consists of basic fuchsin and phenol. It is a dry staining dye for staining acid-fast bacteria such as Mycobacterium tuberculosis. Carbol-Fuchsin solution according to Kinyoun is a modified stain that does not require heat to infuse the carbol-fuchsin into the acid-fast cells during the staining process, and this process is also known as “cold staining”. It requires a higher concentration of the primary stain.

應用

• Kinyoun’s carbol-fuchsin is intended for use to stain paraffin sections of a formaldehyde-fixed specimen containing tubercle bacilli, and for the demonstration of tuberculosis bacteria (Mycobacterium tuberculosis).
• It is also applicable for other organisms that retain the dye after immersion in acidified 70% alcohol.
• It has been used to develop a staining technique for detecting microsporidial spores in fecal specimens.

原則

Acid-fast bacteria possess a waxy, lipid-rich cell wall that absorbs dyes very slowly. To enhance the absorption of carbol-fuchsin dye and the subsequent formation of the mycolate-fuchsin complex, the dye solution applied to the specimen is normally heated to the evaporation point. In Kinyoun’s modification of this method, the heating of the solution is avoided. Consequently, hazardous phenolic vapors are not released.

其他說明

用于显示肺结核菌的染色剂

安全信息

• 象形圖: GHS02,GHS08,GHS05,GHS07
• 訊號詞: Danger
• 危險聲明: H226,H302,H314,H341,H351,H412
• 防範說明: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危險分類: Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: 109.9 °F - closed cup
• 閃點（°C）: 43.3 °C - closed cup