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無菌
sterile; 0.2 μm filtered
品質等級
形狀
solution
包裝
pkg of 10 mL (50x)
製造商／商標名
Roche
濃度
50 ×
技術
cell culture | hybridoma: suitable
雜質
mycoplasma, tested
100 EU/mL endotoxin (LAL test)
溶解度
water: miscible
儲存溫度
−20°C
一般說明
Nutridoma-CS is a serum replacement solution which helps in cell proliferation when added to a suspension. Its major component is β-D xylopyranose which has ring shaped sugar allowing the cells to grow in a suspension even without serum.
應用
Contents
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Biochemically defined supplement for the replacement of fetal bovine serum (FBS) in cultures of hybridoma cells. Nutridoma-CS supports the growth of SP2/0-, P3x63Ag8.653-, and NS-1-derived hybridomas. It is specifically formulated to optimize the growth of freshly fused hybridomas during selection and cloning procedures in serum-free cell culture without feeder cells.
Nutridoma™-CS has been used as a supplement in neurobasal medium for culturing human embryonic stem cell (hESC) lines.
單位定義
Endotoxin (LAL): 100 EU/ml, mycoplasma tested
外觀
Solution (50x concentrated, pH 7.4); sterile. The protein concentration is less than 1 mg/ml for 2% (1x) working concentration.
準備報告
Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol.
其他說明
仅用于生命科学研究。不可用于诊断。
法律資訊
Nutridoma is a trademark of Roche
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
does not flash
閃點（°C）
does not flash
其他客户在看
Debora Steiner et al.
Nature biotechnology, 28(4), 361-364 (2010-03-31)
Undifferentiated human embryonic stem cells (hESCs) are currently propagated on a relatively small scale as monolayer colonies. Culture of hESCs as floating aggregates is widely used for induction of differentiation into embryoid bodies. Here we show that hESC lines can
Mariana Capurro et al.
Gastroenterology, 125(1), 89-97 (2003-07-10)
Early detection of hepatocellular carcinoma (HCC) is critical for successful treatment. However, the differential diagnosis between HCC and benign hepatic lesions is sometimes difficult and new biochemical markers for HCC are required. It has been reported that glypican-3 (GPC3) messenger
Oliver Sterthaus et al.
Cellular reprogramming, 16(3), 185-195 (2014-05-09)
Four normal-karyotype human embryonic stem cell (hESC) lines were generated using the same protocol and maintained under identical conditions. Despite these precautions, gene expression patterns were found to be dissimilar among the four lines. The observed differences were typical of
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