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一般說明
链霉亲和素与辣根过氧化物酶共价结合。结合物可用于检测生物素标记的物质。
應用
链霉亲和素-过氧化物酶(POD)结合物已被用于:
- ELISA
- 原位杂交
- 蛋白质免疫印迹分析
單位定義
一单位链霉亲和素-POD结合物,相当于一单位过氧化物酶,将在+ 25°C和pH 7.0下在1分钟内氧化1 μmol愈创木酚四聚体。
单位换算:1U(+ 25°C;愈创木酚作为底物;pH7.0)对应于1.24 U(+ 25°C;1.7 mM ABTS作为底物;pH = 6.0)或4.0 U(+ 25°C;8.7 mM ABTS作为底物;pH = 5.0)。
单位换算:1U(+ 25°C;愈创木酚作为底物;pH7.0)对应于1.24 U(+ 25°C;1.7 mM ABTS作为底物;pH = 6.0)或4.0 U(+ 25°C;8.7 mM ABTS作为底物;pH = 5.0)。
外觀
液体,稳定化的
準備報告
工作浓度:5 - 500 mU/ml (即储备溶液可稀释)。偶联物的工作浓度取决于应用和底物。以下浓度应作为使用指南:
- ELISA:50至200 mU/ml
- 免疫组织化学:250至1000mU/ml
- 蛋白质印迹:200-250 mU/ml
其他說明
仅用于生命科学研究。不可用于诊断。
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
does not flash
閃點(°C)
does not flash
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