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Merck

11089153001

Roche

链霉亲和素-过氧化物酶结合物

liquid, pkg of 1 mL

别名:

链霉亲和素

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About This Item

分類程式碼代碼:
12352200

形狀

liquid

品質等級

包裝

pkg of 1 mL

製造商/商標名

Roche

儲存溫度

-10 to -25°C

一般說明

链霉亲和素与辣根过氧化物酶共价结合。结合物可用于检测生物素标记的物质。

應用

链霉亲和素-过氧化物酶(POD)结合物已被用于:
  • ELISA
  • 原位杂交
  • 蛋白质免疫印迹分析
它还用于免疫组化。

單位定義

一单位链霉亲和素-POD结合物,相当于一单位过氧化物酶,将在+ 25°C和pH 7.0下在1分钟内氧化1 μmol愈创木酚四聚体。

单位换算:1U(+ 25°C;愈创木酚作为底物;pH7.0)对应于1.24 U(+ 25°C;1.7 mM ABTS作为底物;pH = 6.0)或4.0 U(+ 25°C;8.7 mM ABTS作为底物;pH = 5.0)。

外觀

液体,稳定化的

準備報告

工作浓度:5 - 500 mU/ml (即储备溶液可稀释)。偶联物的工作浓度取决于应用和底物。以下浓度应作为使用指南:
  • ELISA:50至200 mU/ml
  • 免疫组织化学:250至1000mU/ml
  • 蛋白质印迹:200-250 mU/ml

其他說明

仅用于生命科学研究。不可用于诊断。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

does not flash

閃點(°C)

does not flash


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Fernanda V Castro et al.
Cancer immunology, immunotherapy : CII, 61(7), 1005-1018 (2011-12-01)
Effective vaccination against tumour-associated antigens (TAA) such as the 5T4 oncofoetal glycoprotein may be limited by the nature of the T cell repertoire and the influence of immunomodulatory factors in particular T regulatory cells (Treg). Here, we identified mouse 5T4-specific
Angelika Hammerer-Lercher et al.
Clinical chemistry, 54(5), 858-865 (2008-03-15)
The specific forms of pro-B-type natriuretic peptide (proBNP) that occur in human blood are not yet clear. We demonstrated the presence of several proBNP forms in human plasma with a new affinity chromatography method that can be used in combination
Triple-Labeling Whole-Mount In Situ Hybridization Method for Analysis of Overlapping Gene Expression in Brain Tissue with High Level of Autofluorescence.
Chen J
Journal of Cytology & Histology, http://dx-http://dx (2015)
Benjamin Meyer et al.
Frontiers in immunology, 13, 841009-841009 (2022-05-20)
To comprehensively evaluate SARS-CoV-2 specific B-cell and antibody responses up to one year after mild COVID-19. In 31 mildly symptomatic COVID-19 participants SARS-CoV-2-specific plasmablasts and antigen-specific memory B cells were measured by ELISpot. Binding antibodies directed against the proteins spike
Husheem Michael et al.
Frontiers in immunology, 11, 196-196 (2020-03-03)
Human rotavirus (HRV) is a leading cause of morbidity and mortality in children, especially in developing countries. Malnutrition is prevalent in these countries, which may contribute to the decreased oral vaccine efficacy, posing a concern for global health. Neonatal gnotobiotic

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