推荐产品
用途
sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide)
品質等級
製造商/商標名
Roche
環保替代產品特色
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
環保替代類別
, Aligned
運輸包裝
dry ice
一般說明
我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。该产品为一种安全的化学品。 DIG系统是灵敏且具有成本效益的方案,可替代放射性标记和放射法检测核酸。有许多已发表论文证明了DIG系统的通用性,因此,使用放射性标记不再是标记用于杂交的DNA的唯一选择。
第二代寡核苷酸3′末端地高辛标记试剂盒使用了末端转移酶,可将单个地高辛标记双脱氧尿苷三磷酸(ddUTP)催化添加到寡核苷酸的3′-OH端上,从而标记寡核苷酸。
應用
第二代寡核苷酸3′末端地高辛标记试剂盒采用DIG-11-ddUTP和重组末端转移酶对14-100bp的寡核苷酸进行3′-末端标记。
DIG标记寡核苷酸已用于多种杂交技术:
DIG标记寡核苷酸已用于多种杂交技术:
- 斑点/狭缝印迹
- 菌落/斑块杂交
- Southern印迹/Northern印迹
- 原位杂交
特點和優勢
- 由于寡核苷酸具有小分子量,可用于快速杂交动力学
- 单链探针,在杂交过程中不会复性
- 可以根据实验设计序列
- 特别适用于原位杂交;由于寡核苷酸具有小分子量,因此易于扩散到固定的组织和细胞中
包裝
1个试剂盒包含9个组分。
品質
已通过斑点杂交试验测试功能。
原則
利用重组末端转移酶将一个DIG-ddUTP分子添加到寡核苷酸的3′-末端。这能够确保获得具有高特异性和独特性的杂交信号,该信号可利用酶联免疫测定结合抗DIG-AP抗体偶联物以及一种有色或化学发光反应进行检测。
準備報告
活化剂:硫酸钠,Tris
工作浓度:寡核苷酸:100 pmol
在一次标准标记反应中,最多能够标记100pmol(一个30聚体为1 μg)寡核苷酸。
工作浓度:寡核苷酸:100 pmol
在一次标准标记反应中,最多能够标记100pmol(一个30聚体为1 μg)寡核苷酸。
测定时间:从标记寡核苷酸到杂交和检测到第一个可见信号的完整过程,可在24小时内完成。
样品材料
长度为14至100个核苷酸的寡核苷酸,经过HPLC或凝胶电泳纯化
样品材料
长度为14至100个核苷酸的寡核苷酸,经过HPLC或凝胶电泳纯化
儲存和穩定性
-15–-25 °C储存。(未开封试剂盒)
其他說明
仅用于生命科学研究。不可用于诊断。
仅试剂盒组分
产品编号
说明
- Reaction Buffer 5x concentrated
- CoCl<sub>2</sub> Solution 25 mM
- DIG-ddUTP Solution 1 mM
- Recombinant Terminal Transferase 400 U/μl
- Control Oligonucleotide, unlabeled 20 pmol/μl
- Oligonucleotide, DIG-ddUTP labeled 2.5 pmol/μl
- Control DNA, 2.5 pmol/μl pUC 18 DNA, supercoiled
- Glycogen Solution 20 mg/ml
- DNA Dilution Buffer, 50 μg/ml fish sperm DNA
查看所有结果 (9)
訊號詞
Danger
危險分類
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B Inhalation - Repr. 1B
儲存類別代碼
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
水污染物質分類(WGK)
WGK 3
閃點(°F)
does not flash
閃點(°C)
does not flash
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商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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