SCT276

BioTracker^™ Green pH-Independent Autophagy Live Cell Probe

Human

• 别名: autophagosome live cell probe, green AP live cell probe, green autophagosome probe, green autophagy probe
• 分類程式碼代碼: 41106514
• NACRES: NA.47

属性

• product name: BioTracker^™ Green pH-Independent Autophagy Live Cell Probe,
• 生物源: human
• 品質等級: 100
• 包裝: vial of 5 nmol
• 製造商／商標名: Millipore
• 技術: cell culture | stem cell: suitable; flow cytometry: suitable
• 運輸包裝: 2-8°C
• 儲存溫度: 2-8°C

说明

一般說明

SCT276 is a small fluorescent molecule that detects autophagosomes and autolysosomes by a likely mechanism of incorporation into the autophagosome double membrane during its formation, whereupon it emits fluorescence under hydrophobic conditions. This probe has been noted to frequently colocalize with LC3. SPECTRAL CHARACTERISTICS: Excitation λ: 450 nmEmission λ: 535 nm

特點和優勢

Fluorescence microscopyFlow cytometry

標靶描述

Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, dysfunctional proteins aggregate and are enclosed by a double membrane to form an autophagosome.

儲存和穩定性

Store at -20 °C, protected from light.

其他說明

SCT276 detects autophagosomes and autolysosomes by the likely mechanism of incorporation into the autophagosome during double membrane formation, and emits green fluorescence under hydrophobic conditions. SCT276 is cell permeable, does not require transfection, and enables live cell imaging with fluorescence microscopy and quantitative assay by flow cytometry.Add 50 uL DMSO to tube of SCT276 (5 nmol) and pipet to dissolve to make a 0.1 mM stock solution. Dilute with culture medium to 0.1 - 0.5 uM to make working solution.

法律資訊

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable