SCC234

YUMM2.2 Mouse Melanoma Cell Line

Mouse

• 别名: Yale University Mouse Melanoma 2.2 cell line, Yale University Mouse Melanoma cell line
• 分類程式碼代碼: 41106514
• NACRES: NA.81

属性

• product name: YUMM2.2 Mouse Melanoma Cell Line,
• 生物源: mouse
• 品質等級: 100
• 包裝: vial of ≥1x10^6 vial
• 製造商／商標名: Millipore
• 技術: cell culture | mammalian: suitable
• 運輸包裝: liquid nitrogen
• 儲存溫度: −196°C

说明

一般說明

The YUMM (Yale University Mouse Melanoma) cell lines were developed as models that recapitulate genetic drivers found in a significant proportion of human melanomas. YUMM2.2 cells are syngeneic with the immunocompetent C57Bl/6 mouse background.

應用

• Each vial contains > 1X10⁶ viable cells.
• YUMM2.2 cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for interspecies contamination from rat, chinese hamster, Golden Syrian hamster, human and nonhuman primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.

特點和優勢

The YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM2.2 harbors a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis and inactivates wt Pten and Cdkn2a.

標靶描述

The hope for immune-based therapies in cancer and recent progress in successful application of these approaches has focused research on the necessity for immunocompetent models to assess immune system responses to cancer cells. Immunocompetent genetically-engineered mouse models harboring discrete genetic drivers of melanoma are essential for studying potential immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds.The YUMM2.2 cell line harbors a stabilized beta-catenin allele from excision of exon 3, a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis, enhances metastasis, and controls tumor differentiation. Exon 3 excision is analogous to human beta-catenin stabilizing mutations found in human melanomas.SourceThe YUMM2.2 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically engineered mouse model had been introduced via backcrossing. References:1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.

儲存和穩定性

YUMM2.2 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting cell marker expression and functionality.

其他說明

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable