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一般說明
可识别HCT116细胞中的全长APC(p300)和SW480细胞中的截短APC(p147)。抗体靶基因符号:APC 目标同义词:AI047805,Apc7,AU020952,AW124434,BTPS2,DP2,DP2.5,DP3,家族性腺瘤性息肉病,FAP,GS,Min,RATAPC Entrez基因名称:腺瘤性息肉病 Hu Entrez ID:324(有关抗体:OP80,ST1150,OP62,OP47L) Mu Entrez ID:11789 大鼠Entrez ID:24205
抗APC(Ab-1),小鼠单克隆,克隆FE9,可识别HCT116细胞中的全长APC(p300)和SW480细胞中的截短APC(p147)。它已被验证可用于蛋白质印迹法。
蛋白G纯化小鼠单克隆抗体是通过用指定的免疫原免疫小鼠并将脾细胞与SP40细胞融合而产生的。可识别~300 kDa APC蛋白以及多种截短形式。
免疫原
一种与APC N端35个氨基酸对应的合成肽
應用
免疫印迹(1 µg/ml,参见注释)
包裝
请参考特定浓度批号的标签。
警告
毒性:标准处理(A)
外觀
溶于50 mM磷酸钠缓冲液(pH 7.5),含0.2%明胶。
分析報告
阳性对照
HCT116细胞用于p300,SW480细胞用于截短APC(p147)
HCT116细胞用于p300,SW480细胞用于截短APC(p147)
其他說明
Koetsier, P. A., et al. 1993.BioTechniques15, 258.
Smith, K. J., et al. 1993.Proc.Natl.Acad.Sci., USA90, 2846.
Su, L.-K., et al. 1993.Can.Res.53, 2728.
Boynton, R. F., et al. 1992.Proc.Natl.Acad.Sci. USA89, 3385.
D′Amico, D., et al. 1992.Cancer Res.52, 1996.
Fearon, E. R., and Jones, P. A., 1992.FASEB J.6, 2783.
Miyoshi, Y., et al. 1992.Proc.Natl.Acad.Sci. USA89, 4452.
Powell, S. M., et al. 1992.Nature359, 235.
Groden, J., et al. 1991.Cell66, 589.
Kinzler, K. W., et al. 1991.Science253, 661.
Nishisho, I., et al. 1991.Science253, 665.
Smith, K. J., et al. 1993.Proc.Natl.Acad.Sci., USA90, 2846.
Su, L.-K., et al. 1993.Can.Res.53, 2728.
Boynton, R. F., et al. 1992.Proc.Natl.Acad.Sci. USA89, 3385.
D′Amico, D., et al. 1992.Cancer Res.52, 1996.
Fearon, E. R., and Jones, P. A., 1992.FASEB J.6, 2783.
Miyoshi, Y., et al. 1992.Proc.Natl.Acad.Sci. USA89, 4452.
Powell, S. M., et al. 1992.Nature359, 235.
Groden, J., et al. 1991.Cell66, 589.
Kinzler, K. W., et al. 1991.Science253, 661.
Nishisho, I., et al. 1991.Science253, 665.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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