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Key Documents

OP44

Sigma-Aldrich

APC (Ab-1)小鼠单克隆抗体(FE9)

liquid, clone FE9, Calbiochem®

别名:

抗-腺瘤性息肉病性大肠埃希菌

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

FE9, monoclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative

物種活性

rat, human, mouse

製造商/商標名

Calbiochem®

儲存條件

do not freeze

同型

IgG1

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... APC(324)

一般說明

可识别HCT116细胞中的全长APC(p300)和SW480细胞中的截短APC(p147)。
  • 抗体靶基因符号:APC
  • 目标同义词:AI047805,Apc7,AU020952,AW124434,BTPS2,DP2,DP2.5,DP3,家族性腺瘤性息肉病,FAP,GS,Min,RATAPC
  • Entrez基因名称:腺瘤性息肉病
  • Hu Entrez ID:324(有关抗体:OP80ST1150OP62OP47L
  • Mu Entrez ID:11789
  • 大鼠Entrez ID:24205
  • 抗APC(Ab-1),小鼠单克隆,克隆FE9,可识别HCT116细胞中的全长APC(p300)和SW480细胞中的截短APC(p147)。它已被验证可用于蛋白质印迹法。
    蛋白G纯化小鼠单克隆抗体是通过用指定的免疫原免疫小鼠并将脾细胞与SP40细胞融合而产生的。可识别~300 kDa APC蛋白以及多种截短形式。

    免疫原

    一种与APC N端35个氨基酸对应的合成肽

    應用

    免疫印迹(1 µg/ml,参见注释)

    包裝

    请参考特定浓度批号的标签。

    警告

    毒性:标准处理(A)

    外觀

    溶于50 mM磷酸钠缓冲液(pH 7.5),含0.2%明胶。

    分析報告

    阳性对照
    HCT116细胞用于p300,SW480细胞用于截短APC(p147)

    其他說明

    Koetsier, P. A., et al. 1993.BioTechniques15, 258.
    Smith, K. J., et al. 1993.Proc.Natl.Acad.Sci., USA90, 2846.
    Su, L.-K., et al. 1993.Can.Res.53, 2728.
    Boynton, R. F., et al. 1992.Proc.Natl.Acad.Sci. USA89, 3385.
    D′Amico, D., et al. 1992.Cancer Res.52, 1996.
    Fearon, E. R., and Jones, P. A., 1992.FASEB J.6, 2783.
    Miyoshi, Y., et al. 1992.Proc.Natl.Acad.Sci. USA89, 4452.
    Powell, S. M., et al. 1992.Nature359, 235.
    Groden, J., et al. 1991.Cell66, 589.
    Kinzler, K. W., et al. 1991.Science253, 661.
    Nishisho, I., et al. 1991.Science253, 665.

    法律資訊

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    儲存類別代碼

    11 - Combustible Solids

    水污染物質分類(WGK)

    WGK 1

    閃點(°F)

    Not applicable

    閃點(°C)

    Not applicable


    分析证书(COA)

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    访问文档库

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    Mireia Menéndez et al.
    Gastroenterology, 134(1), 56-64 (2008-01-02)
    We identified the APC N1026S variant of unknown malignant potential in the adenomatous polyposis coli (APC) gene in a Spanish attenuated familial adenomatous polyposis (AFAP) family. The variant was located in the first of the 4 highly conserved 15-amino acid
    Shuyuan Zhang et al.
    Cancer research, 74(18), 5311-5321 (2014-07-30)
    Cancer genome sequencing has identified numerous somatic mutations whose biologic relevance is uncertain. In this study, we used genome-editing tools to create and analyze targeted somatic mutations in murine models of liver cancer. Transcription activator-like effector nucleases (TALEN) were designed
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    Scientific reports, 8(1), 16931-16931 (2018-11-18)
    CREB and C/EBP β signaling pathways are modulated during inflammation and also targeted by Bacillus anthracis edema toxin (ET), but how these factors individually and jointly contribute to changes in immune cell function is poorly understood. Using CRISPR/Cas9 gene editing

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