推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
RAS 10, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human, mouse, rat
製造商／商標名
Calbiochem®
儲存條件
do not freeze
同型
IgG2a
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... KRAS(3845)
一般說明
可识别SW480和Y1细胞以及乳腺癌组织中的~21 kDa H-、K-和N-Ras蛋白。
该抗Pan-Ras（Ab-3）小鼠mAb（RAS 10）经验证可用于FC、冰冻切片、免疫印迹、IF、IP和石蜡切片，以检测Pan-Ras（Ab-3）。
通过用指定的免疫原免疫BALB/c小鼠并将脾细胞与SP2/0骨髓瘤细胞融合而产生的纯化小鼠单克隆抗体（请参阅应用参考）。可识别~21 kDa Ras蛋白。
免疫原
重组人p21 Ras
應用
流式细胞术分析（2.5 g/ml）
冷冻切片（2.5 g/ml）
免疫印迹（0.1-3 g/ml，化学发光和比色法，请参阅应用参考文献）
免疫荧光（2.5 g/ml，请参阅应用参考文献）
免疫沉淀（1 g/样品）
石蜡切片（2.5 g/ml，需要皂苷预处理）
包裝
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
分析報告
阳性对照
SW480或Y1细胞或乳腺癌组织
SW480或Y1细胞或乳腺癌组织
其他說明
Rodenhuis, S. et al., 1992.Cancer Res. (Suppl)52, 2665s.
Sidransky, D., et al. 1992.Science256, 102.
Hamer, P.J., et al. 1990.Hybridoma9, 573.
Bos, J.L.1989.Cancer Res.49, 4682.
Furth, M. E., et al. 1987.Oncogene1, 47.
Kraus, M.H., et al. 1984.Proc.Natl.Acad.Sci. USA81, 5384.
Shimizu, K., et al. 1983.Proc.Natl.Acad.Sci. USA80, 2112.
Taparowsky, E., et al. 1983.Cell34, 581.
Ellis, R.W., et al. 1981.Nature292, 506.
Shih, T.Y., et al. 1980.Nature287, 686.
Sidransky, D., et al. 1992.Science256, 102.
Hamer, P.J., et al. 1990.Hybridoma9, 573.
Bos, J.L.1989.Cancer Res.49, 4682.
Furth, M. E., et al. 1987.Oncogene1, 47.
Kraus, M.H., et al. 1984.Proc.Natl.Acad.Sci. USA81, 5384.
Shimizu, K., et al. 1983.Proc.Natl.Acad.Sci. USA80, 2112.
Taparowsky, E., et al. 1983.Cell34, 581.
Ellis, R.W., et al. 1981.Nature292, 506.
Shih, T.Y., et al. 1980.Nature287, 686.
p21ras的表达水平在不同组织中是可变的。因此，我们建议在免疫印迹分析之前进行浓缩步骤，以获得最佳结果。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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