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Key Documents

MHA00MRS2

Millipore

De Man, Rogosa and Sharpe Broth

capacity 2 mL, For final product testing or in-process testing, for lactic acid bacteria, input: beverage(s)

别名:

MRS broth

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About This Item

分類程式碼代碼:
41106225
eCl@ss:
32160801
NACRES:
NA.74

品質等級

無菌

sterile

形狀

liquid

儲存期限

12 mo.

特點

ready-to-use

包裝

pack of 50 tests

容量

2 mL

filling volume

2 mL

顏色

clear red-brown

輸入

sample type beverage(s)
sample type sport drink
sample type cider
sample type soft drink
sample type beer
sample type wine
sample type cosmetics
sample type food(s)
sample type bottled water

pH值

6.5±0.2 (25.0 °C)

應用

clinical
cosmetics
environmental
food and beverages
industrial qc
microbiology
water monitoring

相容性

for use with MF-method
for use with Microfil®
for use with MicropreSure®
for use with Milliflex®

運輸包裝

wet ice

儲存溫度

2-8°C

適合性

Lactobacillus brevis
Lactobacillus fermenti
lactic acid bacteria

一般說明

MRS Broth is used for the enrichment, cultivation and isolation of all species of Lactobacillus. The MRS media formulation was developed by de Man, Rogosa and Sharpe {23} to replace the tomato juice medium and the meat extract tomato juice medium. It is a medium supporting good growth of lactobacilli in general, even those strains which have shown poor growth in existing media, like strains of L. brevis and L. fermenti. The MRS culture media contain polysorbate (Tween 80), acetate, magnesium and manganese which are known to act as special growth factors for Lactobacilli as well as a rich nutrient base. As these media show a very low degree of selectivity Pediococcus and Leuconostoc species as well as other secondary bacteria may grow on them. Most of the accompanying microflora can be inhibited by thallium acetate, sorbic acid, acetic acid, sodium nitrite, cycloheximide and polymyxin. Lactobacillus species appear as small, white, creamy colonies. Saccharomyces species appear as large, white, creamy colonies.{24}

應用

MRS Broth is used for final product testing or in-process testing of beverages for the contamination by lactobacilli, L. brevis and L. fermenti in Beer, Bottled Water, Cider, Soft Drink and Sport Drink. This media finds application in Clinical, Cosmetics and Food and Beverage industry.

培養基

MRS

分析報告

QC organisms
L. casei (ATCC 393)
罗伊氏乳杆菌菌落显示为形体小、白色、乳状菌落。 酿酒酵母菌属将显示为形体大、白色、乳状菌落

法律資訊

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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A medium for the cultivation of lactobacilli
De Man, JC and Rogosa, d M and Sharpe, M Elisabeth
The Journal of Applied Bacteriology, 23(1), 130-135 (1960)
Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams and Wilkins, Baltimore
MacFaddin JF
The Journal of Applied Bacteriology, 23(1), 130-135 (1985)

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