MABT1514

Anti-Tenascin-R Antibody, clone 23-14

• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 23-14, monoclonal
• 分子量: calculated mol wt 148.28 kDa; observed mol wt ~170 kDa
• 純化經由: using protein G
• 物種活性: human, mouse, chicken, rat
• 包裝: antibody small pack of 100 μg
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; inhibition assay: suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: C-terminal half
• Protein ID登錄號: NP_990607
• UniProt登錄號: Q00546
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: chicken ... TNR(396213)

说明

一般說明

Tenascin-R (UniProt: Q00546; also known as TN-R, Restrictin) is encoded by the TNR gene (Gene ID: 396213) in chicken. Tenascins are a family of four large glycoproteins (Tenascin-C, R, X, and W) that share a cysteine-rich N-terminus, a sequence of fibronectin type III domains, and homologies to fibronectin-b at the C-terminus. Tenascin-R (TN-R), the smallest member of the tenascin family that can exist as homodimer or homotrimer. It interacts with different cells and matrix components and is involved in neural cell adhesion and neurite formation. Its interaction with contactin-1 enhances neurite outgrowth. It is expressed weakly in E6 embryo and reaches a maximum at E16 and declines in the adult. Its expression is observed in oligodendrocytes during myelination, and it accumulates at Ranvier nodes and by a subpopulation of neurons, including cerebellar stellate/basket cells, motoneurons in the spinal cord, and hippocampal interneurons. TN-R is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to generate the mature form. Its N-terminal region contains a cysteine-rich segment that it shares with other members of tenascin family. Within this segment there is a stretch of 20 amino acids that form three heptad repeats of hydrophobic amino acids in an a-helix that might generate a triple-stranded coiled coil to link three or two TN-R polypeptides to trimers or dimers. It also contains four EGF-like domains and nine fibronectin type-III (Fn-III) domains. Fn-III 9 mediates cell attachment and Fn-III 2-5 are reported to mediate neurofascin binding. It also contains a fibrinogen C-terminal domain (aa 1124-1339) that mediates its interaction with chondroitin Sulfate Proteoglycan 5. (Ref.: Nörenberg, U., et al. (1995). J. Cell Biol. 130(2); 473-484; Rathjen, FG., et al. (1991). Development.113(1); 151-164).

特異性

Clone 23-14 is a mouse monoclonal antibody that detects Tenascin-R. It targets an epitope within the Fibronectin type-III 8 domain from the C-terminal half.

免疫原

Immunoaffinity isolate of the monoclonal antibody F11 from detergent extracts of adult chicken brain plasma membrane preparations.

應用

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysates.

Western Blotting Analysis: A 1:125 dilution of this antibody detected Tenascin-R in Rat brain tissue lysates.

Tested Applications

Inhibition Assay: A representative lot of this antibody inhibited neural cell and retinal cell attachment to Tenascin-R. (Rathjen, F.G., et al. (1991). Development. 113(1):151-64; Norenberg, U., et al. (1995). J Cell Biol. 130(2):473-84; Norenberg, U., et al. (1996). Int J Dev Neurosci. 14(3):217-31).

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected Tenascin-R in mouse brain and rat brain tissue sections.

Western Blotting Analysis: A representative lot detected Tenascin-R in Western Blotting applications (Wintergerst, E.S., et al. (2001). J Neurocytol. 30(4):293-301).

Immunohistochemistry Analysis: A representative lot detected Tenascin-R in Immunohistochemistry applications (Haunso, A., et al. (2000). Brain Res.;864(1):142-5; Wintergerst, E.S., et al. (2001). J Neurocytol.;30(4):293-301; Geissler, M., et al. (2013). J Neurosci.;33(18):7742-55).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Tenascin-R, clone 23-14, Cat. No. MABT1514, is a mouse monoclonal antibody that detects Tenascin-R and is tested for use in Immunohistochemistry (Paraffin), Inhibition Assay, and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG1 in PBS without azide.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable