推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
FG6-1G, monoclonal
物種活性
human
技術
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG2aκ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... PTPN1(5770)
一般說明
PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and may be a potential therapeutic target for diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.
特異性
This antibody recognizes the catalytic domain of PTP1B.
免疫原
Epitope: Catalytic domain
Recombinant protein corresponding to the catalytic domain of human PTP1B.
應用
Anti-PTP1B Antibody, clone FG6-1G is an antibody against PTP1B for use in Western Blotting, Immunoprecipitation.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Western Blot Analysis: A representative lot was used to detect PTP1B in Jurkat cell lysate.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected PTP1B in A431 cell lysate (Gulati, P. et al. (2004). EMBO Rep. 5(8):812-817.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected PTP1B in A431 cell lysate (Gulati, P. et al. (2004). EMBO Rep. 5(8):812-817.).
品質
Evaluated by Western Blot in TF-1 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PTP1B in 10 µg of TF-1 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PTP1B in 10 µg of TF-1 cell lysate.
標靶描述
~50 kDa observed
聯結
Replaces: 04-1140
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
分析報告
Control
TF-1 cell lysate
TF-1 cell lysate
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
UVA inactivates protein tyrosine phosphatases by calpain-mediated degradation.
Gulati, Pawan, et al.
EMBO Reports, 5, 812-817 (2004)
Giampaolo Minetti et al.
The Biochemical journal, 377(Pt 2), 489-497 (2003-10-07)
One of the most intensively studied post-translational modifications of erythrocyte proteins is the phosphorylation of tyrosine residues of band 3, which is strictly regulated in vivo by PTKs (protein-tyrosine kinases) and PTPs (protein-phosphotyrosine phosphatases). Two PTKs (p72(syk) and p56/53(lyn)) and
Fawaz G Haj et al.
The Journal of biological chemistry, 280(15), 15038-15046 (2005-02-09)
Protein-tyrosine phosphatase 1B (PTP1B) is an important negative regulator of insulin and leptin signaling in vivo. Mice lacking PTP1B (PTP1B-/- mice) are hyper-responsive to insulin and leptin and resistant to diet-induced obesity. The tissue(s) that mediate these effects of global
Xiangxun Chen et al.
Drug design, development and therapy, 15, 171-183 (2021-01-21)
Sodium cantharidinate (SC) has been broadly applied in lung cancer treatment in China, while its specific function in cervical cancer (CC), a great contributor to death of female reproductive system cancers, remains unclear. Our research evaluated the anti-tumor effects of
Avinash D Londhe et al.
Current protocols in chemical biology, 12(3), e84-e84 (2020-08-18)
The reversible oxidation of protein tyrosine phosphatases (PTPs) impairs their ability to dephosphorylate substrates in vivo. This transient inactivation of PTPs occurs as their conserved catalytic cysteine residue reacts with cellular oxidants thereby abolishing the ability of this reactive cysteine
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