MABN640

Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody

clone M98, from rabbit

• 别名: Amyloid Beta, Amyloid B, Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, N-APP2, Soluble APP-alpha, S-APP-alpha, Soluble APP-beta, S-APP-beta, C995.Bet
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: M98, monoclonal
• 物種活性: human
• 物種活性（以同源性預測）: chicken (based on 100% sequence homology), bovine (based on 100% sequence homology), rabbit (based on 100% sequence homology)
• 技術: dot blot: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgGκ
• NCBI登錄號: NP_000475
• UniProt登錄號: P05067
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... APP(351)

说明

一般說明

The protein named Amyloid beta A4, or ABPP, APP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, or Protease nexin II (PN-II) protein and encoded by human gene A4/AD1 is a precursor to 14 peptides some of which are associated with neurological disease such as Alzheimer′s Disease. In pathological conditions Amyloid beta A4 protein is the precursor for the disease associated proteins, Beta amyloid 40, and Beta amyloid 42, major components of plaques in Alzheimer’s Disease. Normally APP plays a role in neuronal development and mediates aspects of axonal transport as well as neurite outgrowth. It is also involved in copper homeostasis/oxidative stress through copper ion reduction in neurons. Amyloid Beta A4 protein is localized to the plasma membrane and Golgi complex until cleaved. Amyloid Beta A4 protein is normally expressed in all fetal tissues and in the adult, frontal cortex expression predominates although there are isoforms widely expressed in non-neuronal cells as well. Normally, amyloid beta A4 is proteolytically processed and the cleavage and secretion of fragments are non-amyloidogenic. However under presenilin/nicastrin mediated processing of the C99 fragment releases beta amyloid proteins that are associated with neurological disease. MABN640 recognizes a specific conformation of residues 2-7 of the amyloid Aß peptide sequence (AEFRHD) found in amyloid fibrils. It does not detectably react with APP, IAPP, a-synuclein or polyQ fibrils A different antibody MABN637, recognizes a specific conformation of residues 3-6 of the amyloid Aß peptide sequence (EFRH) found in amyloid fibrils. It does not recognize pyroglutamyl Aß at residue 3 (1). MABN640 displays a distinct specificity from MABN638 on dot blots of Aß preparations (1). It also reacts with polyQ40 fibrils but not monomers, indicating that it recognizes a generic, sequence independent epitope.

特異性

MABN640 recognizes a specific conformation of residues 2-7 of the amyloid Aß peptide sequence (AEFRHD) found in amyloid fibrils. It does not detectably react with APP, IAPP, a-synuclein or polyQ fibrils (Hatami et al., manuscript in preparation). This antibody reconizes both Abeta40 and ABeta42.

Other homologies: Broad species cross-reactivity is expected.

免疫原

Corresponding to the Beta Amyloid Protein 42 (residue 2-7) of human Amyloid beta fibrils.

Epitope: Beta Amyloid Protein 42 (residue 2-7)

應用

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody is validated for use in Dot Blot and Western Blotting and Immunohistochemistry for the detection of Amyloid beta fibrils.

Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Amyloid beta fibrils in Abeta42 aggregates (Data courtesy of Prof. C. Glabe, UCI).
Immunohistochemistry Analysis: A representative lot detected Amyloid beta fibrils in Frontal cortex of AD patients (Data courtesy of Prof. C. Glabe, UCI).

品質

Evaluated by Dot Blot in Synthetic Abeta40.

Dot Blot Analysis: 0.1 µg/mL of this antibody detected Amyloid beta fibrils in 0.075 µg of Synthetic Abeta40.

外觀

Protein A purified

Format: Purified

Purified rabbit monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable