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生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
4F2, monoclonal
分子量
calculated mol wt 45 kDa
observed mol wt ~48 kDa
純化經由
using protein G
物種活性
mouse, rat, human, chicken
包裝
antibody small pack of 100
技術
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG1κ
表位序列
Unknown
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
基因資訊
rat ... Lhx1(124451)
特異性
Clone 4F2 is a mouse monoclonal antibody that detects.
免疫原
Recombinant rat Lim2/LhxV5 protein.
應用
Quality Control Testing
Evaluated by Western Blotting in Mouse brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Lhx1/5 in Mouse brain tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected Lhx1/5 in Immunocytochemistry applications (Iyer, N.R., et al. (2016). Exp Neurol. 277:305-316).
Immunohistochemistry Applications: A representative lot detected Pan Lhx1/5 in Immunohistochemistry applications (Lu, Y., et al. (2020). Dev Cell. 53(4):473_491.e9; Shirazi Fard, S., et al. (2014). Cell Cycle. 13(3):408-17; Hotani, T., et al. (2018). Mol Ther Oncolytics. 12:162-172; Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40).
Immunofluorescence Analysis: A representative lot detected Pan Lhx1/5 in Immunofluorescence applications (Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40; Samson, A., et al. (2018). Gut. 67(3):562-573).
Western Blotting Analysis: A representative lot detected Pan Lhx1/5 in Western Blotting applications (Hotani, T., et al. (2018). Mol Ther Oncolytics. 12:162-172).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Mouse brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Lhx1/5 in Mouse brain tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected Lhx1/5 in Immunocytochemistry applications (Iyer, N.R., et al. (2016). Exp Neurol. 277:305-316).
Immunohistochemistry Applications: A representative lot detected Pan Lhx1/5 in Immunohistochemistry applications (Lu, Y., et al. (2020). Dev Cell. 53(4):473_491.e9; Shirazi Fard, S., et al. (2014). Cell Cycle. 13(3):408-17; Hotani, T., et al. (2018). Mol Ther Oncolytics. 12:162-172; Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40).
Immunofluorescence Analysis: A representative lot detected Pan Lhx1/5 in Immunofluorescence applications (Yang, M., et al. (2010). J Comp Neurol. 518(14):2818-40; Samson, A., et al. (2018). Gut. 67(3):562-573).
Western Blotting Analysis: A representative lot detected Pan Lhx1/5 in Western Blotting applications (Hotani, T., et al. (2018). Mol Ther Oncolytics. 12:162-172).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
LIM/homeobox protein Lhx5 (UniProt: P61376; also known as LIM homeobox protein 5, Homeobox protein LIM-2) is encoded by the Lhx5 (also known as Lim-2, Lim2) gene (Gene ID: 124451) in rat. Lhx5 is a nuclear protein that belongs to a family of proteins that carry the LIM domain. Its LIM zinc-binding domains are localized in amino acids 3-61 and 62-125. It acts as a transcriptional regulator and is reported to play an essential role in the regulation of neuronal differentiation and migration during development of the central nervous system. Its expression is detected in developing Purkinje cells soon after they exited the cell cycle and migrate out of the cerebellar ventricular zone. Lhx5 is also required for the generation of the mamillary body (MM) and its derived tracts that are related to memory and spatial navigation that interconnect hippocampal, thalamic, and tegmental regions. It is predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Lhx5 is reported to regulate the local inhibition of Wnt proteins. Activation of Lhx5 is shown to expand forebrain structures. It can rescue forebrain deficiencies caused by excess Wnt activity. Its inhibition results in ectopic activation of Wnt signaling. It regulates the expression of two secreted Frizzled-related Wnt antagonists, Sfrp1a and Sfrp5 that can reduce the ectopic activation of Wnt signaling and rescue the forebrain deficiencies caused by inhibition of Lhx5 function. (Ref.: Miquelajáuregui, A., et al. (2015) Front. Neuroanat. 9; 136; Yang, M., et al. (2010). J. Comp. Neurol. 518(14); 2818-2840; Peng, G., and Westerfield, M. (2006). Development. 133(16); 3191-3200).
外觀
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
重構
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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