MABN2443

Anti-nonphospho-GSK3 (Ser9) Antibody, beta specific Antibody, clone 12B2

clone 12B2, from mouse

• 别名: Glycogen synthase kinase-3 beta, EC: 2.7.11.26, GSK-3 beta, Serine/threonine-protein kinase GSK3B
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 12B2, monoclonal
• 物種活性: rat, human, mouse
• 包裝: antibody small pack of 25 μL
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_002084.2
• UniProt登錄號: P49841
• 運輸包裝: ambient
• 目標翻譯後修改: phosphorylation (pSer9)
• 基因資訊: human ... GSK3B(2932)

说明

一般說明

Glycogen synthase kinase-3 beta (UniProt: P49841; also known as EC: 2.7.11.26, GSK-3 beta, Serine/threonine-protein kinase GSK3B) is encoded by the GSK3B gene (Gene ID: 2932) in human. GSK-3 is a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways during embryogenesis and in metabolic control. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3 alpha and a 47 kDa GSK-3 beta. The GSK-3 alpha contains a glycine-rich extension at its N-terminal. GSK-3 is constitutively active in cells and is regulated through inhibition of its activity. GSK-3 shows a preference for target proteins that are pre-phosphorylated at a `priming residue located C-terminal to the site of GSK-3 phosphorylation. Priming phosphorylation, although not absolutely required, enhances the efficiency of phosphorylation of most GSK-3 substrates. Phosphorylation of a threonine residue in the activation loop (T-loop) is considered to be essential for its activity. The T-loop of GSK-3 beta is phosphorylated at Tyr216, which play a role in forcing open the substrate-binding site of the enzyme. A primary mechanism of GSK-3 beta activity regulation is phosphorylation of N-terminal serine residues (Serine 9). Phosphorylation at this site is inhibitory to GSK-3 beta kinase activity because the phosphorylated N-terminus acts as a competitive inhibitor for primed substrates. GSK-3 beta is constitutively active in resting cells and treatment of cells with an agent, such as insulin, causes GSK-3 inactivation through a PI 3-kinase (PI 3-K)-dependent mechanism.

特異性

Clone 12B2 detects GSK-3 beta in human, murine, and rat cells.

免疫原

Epitope: N-terminus

KLH-conjugated three linear peptides from the N-terminal region of human GSK-3 beta.

應用

Anti-nonphospho-GSK3 (Ser9), beta specific, clone 12B2, Cat. No. MABN2443, is a highly specific mouse monoclonal antibody that targets Glycogen synthase kinase-3 beta and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:500 dilution from a representative lot detected nonphospho-GSK3 (Ser9) in human and mouse brain tissue lysates.

ELISA Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in ELISA applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Western Blotting Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected nonphospho-GSK3 (Ser9) in human cerebral cortex tissue.

Immunocytochemistry Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Immunoprecipitation Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Immunohistochemistry Analysis: A representative lot detected nonphospho-GSK3 (Ser9) in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

品質

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected nonphospho-GSK3 (Ser9) in rat brain tissue lysate.

標靶描述

~45 kDa observed. 46.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: does not flash
• 閃點（°C）: does not flash