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Merck
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Key Documents

MABN153

Sigma-Aldrich

Anti-HuC/HuD Antibody, clone 15A7.1

clone 15A7.1, from mouse

别名:

ELAV-like protein 3, Hu-antigen C, HuC, Paraneoplastic cerebellar degeneration-associated antigen, Paraneoplastic limbic encephalitis antigen 21, ELAV-like protein 4, Hu-antigen D, HuD, Paraneoplastic encephalomyelitis antigen HuD

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

15A7.1, monoclonal

物種活性

human, rat, mouse

技術

immunohistochemistry: suitable (paraffin)
western blot: suitable

同型

IgG2aκ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... ELAVL4(1996)

一般說明

Hu proteins are mammalian embryonic lethal abnormal visual system (ELAV)-like neuronal RNA-binding proteins that contain three RNA recognition motifs. RNA binding protein HuC promotes neuronal development in both the central and the peripheral nervous systems. HuC antibodies are used as markers for developing neurons. HuD may play a role in neuron-specific RNA processing, and be involved in maintaining the proliferative state of cells.

免疫原

KLH-conjugated linear peptide corresponding to human HuC/HuD.

應用

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected HuC/HuD in human brain and rat hippocampus tissues.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This HuC/HuD antibody is validated for use in WB & IHC for the detection of the HuC/HuD protein.

品質

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HuC/HuD in 200 µg of human brain tissue lysate.

標靶描述

~ 40 kDa observed. An uncharacterized band may appear at ~45 kDa in some lysates.

外觀

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Human brain tissue lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Yaxing Gui et al.
Brain pathology (Zurich, Switzerland), 30(1), 151-164 (2019-07-06)
The 18 kDa translocator protein (TSPO) is a widely used target for microglial PET imaging radioligands, but its expression in post-mortem normal and diseased human brain is not well described. We aimed at characterizing the TSPO expression in human control

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