推荐产品
生物源
rat
共軛
FITC conjugate
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
30-F11, monoclonal
物種活性
mouse
技術
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG2bκ
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... PTPRC(5788)
一般說明
CD45, also known as PTPRC, is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells. CD 45 belongs to the protein-tyrosine phosphatase family, receptor class 1/6 subfamily.It also contains 2 fibronectin type-III domains and 2 tyrosine-protein phosphatase domains. CD45 has been shown to play an important role in T-cell activation through the antigen receptor. CD45 also is suggested to act as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one is suggested to affect the substrate specificity of the first domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN. Furthermore, it has been shown to dephosphorylate LYN, and modulate LYN activity. Interestingly, CD45 has been associated with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. CD45 has been shown to bind with GANAB and PRKCSH. Additionally, it interacts with SKAP1 and DPP4, whereas the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Mutations in CD45 have been associated with Multiple Sclerosis (MS) and severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
免疫原
Mouse thymus or spleen
應用
Anti-CD45 (mouse) Antibody, FITC, clone 30-F11 is a highly specific rat monoclonal antibody, that targets CD45 & has been tested in Flow Cytometry, IHC, western blotting & IP.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Cytokines & Cytokine Receptors
Western Blotting Analysis: A representative lot detected human CD45 in Western Blotting. (Tsuboi S and Fukuda M. 1998)
Immunohistochemistry Analysis: A representative lot detected human CD45 in Immunohistochemistry. (Simon DI, et al. 2000; Cornet A, et al. 2001)
Immunoprecipitation Analysis: A representative lot detected human CD45 in Immunoprecipitation. (Ledbetter JA, et al. 1979)
Immunohistochemistry Analysis: A representative lot detected human CD45 in Immunohistochemistry. (Simon DI, et al. 2000; Cornet A, et al. 2001)
Immunoprecipitation Analysis: A representative lot detected human CD45 in Immunoprecipitation. (Ledbetter JA, et al. 1979)
品質
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Flow Cytometry Analysis: 0.25 μg of this antibody detected CD45 in one million C57BL/6 mouse splenocytes.
Flow Cytometry Analysis: 0.25 μg of this antibody detected CD45 in one million C57BL/6 mouse splenocytes.
標靶描述
145 kDa calculated
外觀
Affinity
Purified rat conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.
儲存和穩定性
The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
Binhui Zhou et al.
Frontiers in immunology, 11, 607442-607442 (2021-01-26)
Zdhhc family genes are composed of 24 members that regulate palmitoylation, a post-translational modification process for proteins. Mutations in genes that alter palmitoylation or de-palmitoylation could result in neurodegenerative diseases and inflammatory disorders. In this study, we found that Zdhhc2
Overexpression of branched O-linked oligosaccharides on T cell surface glycoproteins impairs hu?moral immune responses in transgenic mice.
Tsuboi, S and Fukuda, M
The Journal of Biological Chemistry, 273, 30680-30687 (1998)
D I Simon et al.
The Journal of clinical investigation, 105(3), 293-300 (2000-02-17)
Inflammation plays an essential role in the initiation and progression of atherosclerosis, but its role in vascular repair after mechanical arterial injury (i.e., percutaneous transluminal coronary angioplasty, PTCA) is unknown. In animal models of vascular injury, leukocytes are recruited as
A Cornet et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(23), 13306-13311 (2001-11-01)
Early pathological manifestations of Crohn's disease (CD) include vascular disruption, T cell infiltration of nerve plexi, neuronal degeneration, and induction of T helper 1 cytokine responses. This study demonstrates that disruption of the enteric glial cell network in CD patients
Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.
J A Ledbetter et al.
Immunological reviews, 47, 63-90 (1979-01-01)
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