MABF3107

Anti-ULBP2 Antibody, clone BUMO1

• 别名: ALCAN-alpha, N2DL-2, NKG2D ligand 2, NKG2DL2, Retinoic acid early transcript 1H, UL16-binding protein 2
• 分類程式碼代碼: 12352203

属性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: BUMO1, monoclonal
• 分子量: calculated mol wt 27.37 kDa; observed mol wt ~N/A kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 100
• 技術: ELISA: suitable; flow cytometry: suitable
• 同型: IgG1κ
• 表位序列: Unknown
• Protein ID登錄號: NP_079493
• UniProt登錄號: Q9BZM5
• 儲存溫度: 2-8°C
• 基因資訊: human ... ULBP2(80328)

说明

特異性

Clone BUMO1 is a mouse monoclonal antibody that detects UL16-binding protein 2 (ULBP2).

免疫原

p815 murine mastocytoma cells expressing human ULBP2.

應用

Quality Control Testing

Evaluated by Flow Cytometry in C1R cells expressing ULBP2.

Flow Cytometry Analysis: 1.0 µg of this antibody detected ULBP2 in one million C1R cells expressing ULBP2.

Tested Applications

ELISA Analysis: A representative lot detected ULBP2 in ELISA applications (Waldhauer, I., et al. (2006). Cancer Res. 66(5):2520-6; Weil, S., et al. (2017). Front Immunol. 10:8:387).

Flow Cytometry Analysis: A representative lot detected ULBP2 in Flow Cytometry applications (Welte, S.A., et al. (2003). Eur J Immunol. 33(1):194-203; Waldhauer, I., et al. (2006). Cancer Res. 66(5):2520-6; Heinemann, A., et al. (2012). Cancer Res. 72(2):460-71).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

UL16-binding protein 2 (UniProt: Q9BZM5; also known as ALCAN-alpha, NKG2D ligand 2, N2DL-2, NKG2DL2, Retinoic acid early transcript 1H) is encoded by the ULBP2 (also known as N2DL2, RAET1H, UNQ463/PRO791) gene (Gene ID: 80328) in human. The UL16-binding proteins (ULBPs) belong to the family of MHC class I-related molecules that are targets of human CMV glycoprotein. ULBP2 is a GPI-anchor glycoprotein that is synthesized with a signal peptide (aa 1-25) and a propeptide (aa 217-246), which are subsequently cleaved off to produce the mature form. ULBP2 serves as a ligand of the activating immunoreceptor NKG2D and mediates natural kill (NK) cell cytotoxicity. It is detected in cell membrane and endoplasmic reticulum. A secreted for has also been described. Its expression is observed in cancer cells and in the fetus, but not in normal tissues. Tumor cells are reported to release the soluble form of ULBP2 by the action of metalloproteases, however, unlike membrane bound form, it does not down-regulate NKG2D on NK cells. Soluble form of ULBP2 is frequently detected in sera of patients with hematopoietic malignancies. NKG2D, frequently expressed on tumor cells, stimulate tumor immunity. Soluble forms of ULBPs are also reported to induce protein tyrosine phosphorylation, activation of Janus kinase 2, MAP kinase, and PI-3 kinase/Akt signaling. Clone BUMO-1 specifically detects ULBP2 and is suitable for multiple immunodetection applications. (Ref.: Waldhauer, I., and Steinle, A. (2006). Cancer Res 66(5); 2520-2526; Welte, SA., et al. (2003). Eur. J. Immunol. 33(1); 194-203; Sutherland, CL., et al. (2002). J. Immunol. 168(2); 671-679).

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

重構

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.