生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
DN2, monoclonal
分子量
observed mol wt ~80 kDa
純化經由
using protein G
物種活性
human
物種活性（以同源性預測）
rabbit
包裝
antibody small pack of 100
技術
ELISA: suitable
immunohistochemistry: suitable
multiplexing: suitable
western blot: suitable
同型
IgG1κ
表位序列
N-terminal
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
基因資訊
rabbit ... PADI2(100357368)
特異性
Clone DN2 is a mouse monoclonal antibody that detects Protein-arginine deiminase 2 (PADI2/PAD2). It targets an epitope within the N-terminal region.
免疫原
Protein-arginine deiminase (PADI2/PAD2) from rabbit skeletal muscle.
應用
Quality Control Testing
Evaluated by Western Blotting with full-length, recombinant, Human PADI2/PAD2.
Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, recombinant, Human PADI2/PAD2.
Tested Applications
Immunohistochemistry Applications: A representative lot detected PADI2/PAD2 in Immunohistochemistry applications (Damgaard, D., et al. (2014). J Immunol Methods. 405:15-22).
Western Blotting Analysis: A representative lot detected PADI2/PAD2 in Western Blotting applications (Damgaard, D., et al. (2014). J Immunol Methods. 405:15-22; Bawadekar, M., et al. (2017). J Autoimmun. 80:39-47).
ELISA Analysis: A representative lot detected PADI2/PAD2 in ELISA applications (Damgaard, D., et al. (2014). J Immunol Methods.;405:15-22; Damgaard, D., et al. (2016). Rheumatology (Oxford). 55(5):918-27).
Multiplexing: A representative lot detected PADI2/PAD2 in Multiplexing applications (Vejlstrup, A., et al. (2019). J Inflamm Res. 29:12:137-144).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with full-length, recombinant, Human PADI2/PAD2.
Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, recombinant, Human PADI2/PAD2.
Tested Applications
Immunohistochemistry Applications: A representative lot detected PADI2/PAD2 in Immunohistochemistry applications (Damgaard, D., et al. (2014). J Immunol Methods. 405:15-22).
Western Blotting Analysis: A representative lot detected PADI2/PAD2 in Western Blotting applications (Damgaard, D., et al. (2014). J Immunol Methods. 405:15-22; Bawadekar, M., et al. (2017). J Autoimmun. 80:39-47).
ELISA Analysis: A representative lot detected PADI2/PAD2 in ELISA applications (Damgaard, D., et al. (2014). J Immunol Methods.;405:15-22; Damgaard, D., et al. (2016). Rheumatology (Oxford). 55(5):918-27).
Multiplexing: A representative lot detected PADI2/PAD2 in Multiplexing applications (Vejlstrup, A., et al. (2019). J Inflamm Res. 29:12:137-144).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Protein-arginine deiminase (UniProt: A0A5F9DB16; also known as PADI2, PAD2) is encoded by the PADI2 gene (Gene ID: 100357368) in rabbit. Protein-arginine deiminases (PAD) catalyze the hydrolysis of peptidyl-arginine to peptidyl-citrulline and alter protein function. Five isoforms of PAD have been described in mammals (PAD1, 2, 3, 4, and 6) that are homologous in their catalytic site but are expressed differently in different cells and tissues and have different physiological roles. PAD2, one of the most abundant PADs has three domains: N-terminal domain (aa 1-114), a central domain (aa 116-274), and a C-terminal domain (aa 285-657). High calcium concentration is reported to be a prerequisite for PAD2 activation. A redox potential maintains its catalytic site in a reduced state. Overexpression of PAD2 has been associated with inflammatory diseases, such as rheumatoid arthritis and neurodegenerative diseases including multiple sclerosis. Higher levels of PAD2 are observed in transformed cell model of breast cancer progression with increasing tumor malignancy and in a murine models of xenograft tumors. Clone DN2 binds to an epitope in the N-terminal region and is ideally suited for quantitative ELISA. (Ref.: Wang, Y., et al. (2021). Int. J. Cancer. 148(2); 267-276; Vejlstrup, A., et al. (2019). J. Inflamm. Res. 12; 137 144; Damgaard, D., et al. (2016). Rheumatology 55(5); 918-927; Damgaard, D., et al. (2014). J. Immunol. Methods. 405; 15-22).
外觀
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
重構
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门