推荐产品
生物源
rat
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
JUAM71D, monoclonal
分子量
calculated mol wt 64.61 kDa
observed mol wt ~64 kDa
純化經由
using protein G
物種活性
human
包裝
antibody small pack of 100 μL
技術
immunofluorescence: suitable
western blot: suitable
同型
IgG2b
表位序列
Unknown
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
基因資訊
human ... PRIMPOL(201973)
一般說明
DNA-directed primase/polymerase protein (UniProt: Q96LW4; also known as hPrimpol1, Coiled-coil domain-containing protein 111) is encoded by the PRIMPOL (also known as CCDC111) gene (Gene ID: 201973) in human. PrimPol is an enzyme with both DNA primase and DNA polymerase activities and is involved in DNA damage tolerance. It is detected in nuclei and cytosol with a large fraction localized inside the mitochondria. Upon UV irradiation, it is rapidly redirected to chromatin to reprime DNA synthesis. It associates with nuclear chromatin during the G1 and S phases of unperturbed cell cycles. It contains two RPA1-binding motifs (RBM) (aa 513-527 and 548-556) of which RPA1 is shown to be essential for recruitment to chromatin. It binds to the basic cleft of RPA1. The RPA2-binding motif plays a supporting role in RPA1-binding. PrimPol is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase and is able to bypass certain DNA lesions. It displays high capacity to tolerate DNA damage lesions and is able to synthesize DNA primers downstream of lesions and allows DNA replication to continue. It is also shown to realign primers ahead of ′unreadable lesions′ thereby skipping the lesion. In vitro, PrimPol is reported to act as a DNA primase, elongating primers that PrimPol itself synthesizes, or as translesion synthesis DNA polymerase that elongates pre-existing primers across lesions. In murine models PrimPol knockout cells display hypersensitivity to DNA interstrand crosslinks-inducing drugs. Two isoforms of PrimPol have been described that are produced by alternative splicing. (Ref.: Diaz-Talavera, I., et al. (2022). Biomolecules. 12(2); 248; Gonzales-Acosta, D., et al. (2021). EMBO J. 40(14); e106355; Guilliam, TA., et al. (2017). Nat. Commun. 8:15222; Garcia-Gomez, S., et al. (2013). Mol. Cell. 52(4); 541-553; Mouron, S., et al. (2013). Nat. Struct. Mol. Biol. 20(12); 1383-1389).
特異性
Clone JUAM71D is a rat monoclonal antibody that detects DNA-directed primase/polymerase (PrimPol).
免疫原
His-tagged, full-length, recombinant human DNA-directed primase/polymerase (PrimPol).
應用
Quality Control Applications
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PrimPol in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A representative lot detected PrimPol in Western Blotting applications (Gonzalez-Acosta, D., et al. (2021). EMBO J ;40(14); e106355).
Immunofluorescence Analysis: A representative lot detected PrimPol in Immunofluorescence applications (Gonzalez-Acosta, D., et al. (2021). EMBO J. 40(14); e106355).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PrimPol in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A representative lot detected PrimPol in Western Blotting applications (Gonzalez-Acosta, D., et al. (2021). EMBO J ;40(14); e106355).
Immunofluorescence Analysis: A representative lot detected PrimPol in Immunofluorescence applications (Gonzalez-Acosta, D., et al. (2021). EMBO J. 40(14); e106355).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
外觀
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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