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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies, primary antibodies
無性繁殖
A4, monoclonal
分子量
calculated mol wt 79.15 kDa
observed mol wt ~79 kDa
純化經由
using protein G
物種活性
rat, human, mouse
包裝
antibody small pack of 100 μg
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG
表位序列
N-terminal
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
rat ... Cpeb4(303010)
一般說明
Cytoplasmic polyadenylation element binding protein 4 (UniProt: D3ZKL3; also known as CPEB4) is encoded by the Cpeb4 (also known as rCG_33472) gene (Gene ID: 303010) in rat. The CPEB family of RNA-binding proteins regulate translation in various tissues in vertebrates. They shuttle between nucleocytoplasmic compartments with longer retention in the cytoplasm where they regulate target RNA translation. CPEB4 is an RNA-binding protein that promotes polyadenylation and translation of target RNAs and contributes to meiotic and mitotic progression. It acts as a survival factor exclusively for early postnatal granule cells in the olfactory bulb. CPEB4 is reported to interact with adaptor protein vinexin by binding to its first two SH3 domains and this interaction leads to vinexin localization in stress granules. It is shown that CPEB4 recruits vinexin to stress granules in response to adverse conditions, such as heat shock or arsenite-induced stress. CPEB4 is also shown to be essential for cell survival and becomes nuclear in response to focal ischemia in vivo and when cultured neurons are deprived of oxygen and glucose. (Ref.: Tseng, CS., et al. (2017). Cell Reports 21, 2264-2276; Chang, YW., and Huang, YS. (2014). PLoS ONE 9(9); e107961; Kan, MC., et al. (2010). Mol. Cell Biol. 30(24); 5658-5671).
特異性
Clone A4 is a mouse monoclonal antibody that detects Cytoplasmic polyadenylation element binding protein 4 (CPEB4). It targets an epitope within the N-terminal region.
免疫原
Recombinant fragment corresponding to the first 458 amino acids from the N-terminal region of rat CPEB4.
應用
Quality Control Testing
Evaluated by Western Blotting in Neuro-2A cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CPEB4 in Neuro-2A cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CPEB4 in A375 and HepG2 cell lysates.
Immunoprecipitation Analysis: A representative lot immunoprecipitated CPEB4 in Immunoprecipitation applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961).
Immunocytochemistry Analysis: A representative lot detected CPEB4 in Immunocytochemistry applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961).
Western Blotting Analysis: A representative lot detected CPEB4 in Western Blotting applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961; Tseng, C-S., et al. (2017). Cell Rep. 21(8):2264-2276).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Neuro-2A cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CPEB4 in Neuro-2A cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CPEB4 in A375 and HepG2 cell lysates.
Immunoprecipitation Analysis: A representative lot immunoprecipitated CPEB4 in Immunoprecipitation applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961).
Immunocytochemistry Analysis: A representative lot detected CPEB4 in Immunocytochemistry applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961).
Western Blotting Analysis: A representative lot detected CPEB4 in Western Blotting applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961; Tseng, C-S., et al. (2017). Cell Rep. 21(8):2264-2276).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CPEB4, clone A4, Cat. No. MABE1915, is a mouse monoclonal antibody that detects CPEB4 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
外觀
Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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