推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
28G9, monoclonal
物種活性
mouse, rat, human
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... CREB3L2(64764)
一般說明
Cyclic AMP-responsive element-binding protein 3-like protein 2 (UniProt Q8BH52; also known as cAMP-responsive element-binding protein 3-like protein 2) is encoded by the Creb3l2 (also known as Bbf2h7, C530025K05Rik, SCIRR69) gene (Gene ID 208647) in murine species. Creb3l2/Bbf2h7 belongs to the old astrocyte specifically induced substance (OASIS) family of ER-resident transcription factors that function as ER stress transducers. OASIS members are ER transmembrane proteins that possess both a transcription-activation domain and a basic leucine zipper (bZIP) domain. Upon initial activation through regulated intramembrane proteolysis (RIP), OASIS family members are transported from the ER to the Golgi apparatus. Sequential cleavage by site-1 protease (S1P) and site-2 protease (S2P) releases the N-terminal fragment, which then translocates to the nucleus and activates the transcription of target genes. Under normal conditions, cellular BBF2H7 and OASIS levels are low due to degradation via the ubiquitin-proteasome pathway as a result of constant E3 ubiquitin ligase HRD1- (HMG-CoA reductase degradation 1-) mediated ubiquitination. ER stress is shown to disrupt their interaction with HRD1, resulting in enhanced stability of BBF2H7 and OASIS and an upregulation of their target genes.
免疫原
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse BBF2H7/CREB3L2.
應用
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-BBF2H7/CREB3L2 Antibody, clone 28G9 is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of BBF2H7/CREB3L2.
Western Blotting Analysis: 0.25 µg/mL from a representative lot detected BBF2H7/CREB3L2 in 10 µg of mouse MC3T3-E1 preosteoblasts.
Immunoprecipitation: A representative lot co-immunoprecipitated ER-associated E3 Ub ligase Hrd1 with BBF2H7/CREB3L2 from rat C6 glioma cells only before, but not after, ER stress induction by thapsigargin treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
Western Blottting Analysis: A representative lot detected BBF2H7/CREB3L2 upregulation in murine ATDC5 chondrogenic cells after siRNA-mediated Hrd1 knockdown, as well as BBF2H7/CREB3L2 upregulation in HeLa cells upon proteasome inhibition or ER stress induction (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949.)
Immunocytochemistry: A representative lot detected enhanced ER & nuclear BBF2H7/CREB3L2 immunoreactivity in rat glioma C6 cells upon proteasome inhibitor MG132 treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
Immunoprecipitation: A representative lot co-immunoprecipitated ER-associated E3 Ub ligase Hrd1 with BBF2H7/CREB3L2 from rat C6 glioma cells only before, but not after, ER stress induction by thapsigargin treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
Western Blottting Analysis: A representative lot detected BBF2H7/CREB3L2 upregulation in murine ATDC5 chondrogenic cells after siRNA-mediated Hrd1 knockdown, as well as BBF2H7/CREB3L2 upregulation in HeLa cells upon proteasome inhibition or ER stress induction (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949.)
Immunocytochemistry: A representative lot detected enhanced ER & nuclear BBF2H7/CREB3L2 immunoreactivity in rat glioma C6 cells upon proteasome inhibitor MG132 treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
品質
Evaluated by Western Blotting in rat C6 glioma cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected BBF2H7/CREB3L2 in 10 µg of rat C6 glioma cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected BBF2H7/CREB3L2 in 10 µg of rat C6 glioma cell lysate.
標靶描述
~57-60 kDa observed. Uncharacterized band(s) may appear in some lysates.
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
S Kondo et al.
Cell death and differentiation, 19(12), 1939-1949 (2012-06-19)
Endoplasmic reticulum (ER) stress transducers transduce signals from the ER to the cytoplasm and nucleus when unfolded proteins accumulate in the ER. BBF2 human homolog on chromosome 7 (BBF2H7) and old astrocyte specifically induced substance (OASIS), ER-resident transmembrane proteins, have
Daniele Pittari et al.
Frontiers in cell and developmental biology, 10, 986997-986997 (2022-11-01)
Upon progesterone stimulation, Endometrial Stromal Cells (EnSCs) undergo a differentiation program into secretory cells (decidualization) to release in abundance factors crucial for embryo implantation. We previously demonstrated that decidualization requires massive reshaping of the secretory pathway and, in particular, of
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门