推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
3F2/C6, monoclonal
物種活性
human
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... LIMD1(8994)
一般說明
LIMD1 (LIM domains-containing protein 1) is a tumor suppressor and member of the Ajuba family of LIM domain-containing proteins. LIMD1 interacts with retinoblastoma protein (pRB), inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Recently, LIMD1 has been implicated in regulating cellular processes involved in hypoxia. LIMD1 acts as a molecular scaffold, simultaneously binding the prolyl hydroxylases (PHDs) and von Hippel Lindau tumor suppressors (VHL), thereby assembling a PHD-LIMD1-VHL protein complex and creating an enzymatic niche that enables efficient degradation of HIF-1α.
特異性
This antibody recognizes human LIMD1.
免疫原
Recombinant protein corresponding to human LIMD1
應用
Detect LIMD1 using this Anti-LIMD1 Antibody, clone 3F2/C6 validated for use in ICC, IHC, Immunoprecipitation, Western Blotting.
Research Category
Apoptosis & Cancer
Stem Cell Research
Apoptosis & Cancer
Stem Cell Research
Research Sub Category
Oncoproteins
Oxidative Stress
Oncoproteins
Oxidative Stress
This antibody has been shown to work in Immunocytochemical applications (Foxler, D., et al. (2012).
This antibody has been shown to work in Immunohistochemical applications (Sharp, T., et al. (2008).
This antibody has been shown to work in Immunoprecipitation applications (Courtesy of Dr. Tyson Sharp, Cancer Research, UK)
This antibody has been shown to work in Immunohistochemical applications (Sharp, T., et al. (2008).
This antibody has been shown to work in Immunoprecipitation applications (Courtesy of Dr. Tyson Sharp, Cancer Research, UK)
品質
Evaluated by Western Blot in H1299 cell lysates.
Western Blot Analysis: A 1:2000 dilution of this antibody detected LIMD1 in H1299 cell lysates.
Western Blot Analysis: A 1:2000 dilution of this antibody detected LIMD1 in H1299 cell lysates.
標靶描述
~72 kDa observed
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
分析報告
Control
H1299 cell lysates.
H1299 cell lysates.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Daniel E Foxler et al.
Nature cell biology, 14(2), 201-208 (2012-01-31)
There are three prolyl hydroxylases (PHD1, 2 and 3) that regulate the hypoxia-inducible factors (HIFs), the master transcriptional regulators that respond to changes in intracellular O(2) tension. In high O(2) tension (normoxia) the PHDs hydroxylate two conserved proline residues on
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