MABC1762

Anti-COPS5 Antibody

purified antibody, clone 4-9E, from mouse

• 分類程式碼代碼: 12352203
• NACRES: NA.41
• 無性繁殖: 4-9E, monoclonal
• application: IHC (p); IP; WB
• 物種活性: mouse, human
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable

属性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4-9E, monoclonal
• 分子量: calculated mol wt 37.58 kDa; observed mol wt ~35 kDa
• 物種活性: mouse, human
• 包裝: antibody small pack of 100 μg
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG
• 運輸包裝: dry ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

说明

特異性

Clone 4-9E is a mouse monoclonal antibody that detects COP9 signalosome complex subunit 5 (COPS5). It targets an epitope within the N-terminal region.

免疫原

His-tagged recombinant fragment corresponding to the first 198 amino acids from human COP9 signalosome complex subunit 5 (COPS5).

應用

Quality Control TestingEvaluated by Western Blotting in HEK293T cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected COPS5 in HEK293T cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected COPS5 in NIH3T3 cell lysate.Immunoprecipitation Analysis: A representative lot immunoprecipitated COPS5 in Immunoprecipitation application (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).Immunohistochemistry (Paraffin) Analysis: A representative lot detected COPS5 in Immunohistochemistry application (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).Western Blotting Analysis: A representative lot detected COPS5 in Western Blotting application (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-COPS5, clone 4-9E, Cat. No. MABC1762, is a mouse monoclonal antibody that detects COPS5 and is tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

標靶描述

COP9 signalosome complex subunit 5 (UniProt: Q92905; also known as SGN5, Signalosome subunit 5, Jun activation domain-binding protein 1, COPS5) is encoded by the COPS5 (also known as CSN5, JAB1) gene (Gene ID: 10987) in human. COPS5 is the fifth component of the COP9 signalosome (CSN) complex that directly interacts with and regulates the activity of cellular and developmental processes, including checkpoint control, DNA repair, apoptosis, and cell cycle control. This complex is an essential regulator of the ubiquitin conjugation pathway that mediates the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to a reduction in ubiquitin ligase activity of SCF-type complexes. In the CSN complex, COPS5 probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. However, it lacks the metalloproteinase activity and requires the other subunits of CSN complex. COPS5 is present both in the nucleus and cytoplasm. Its nuclear localization is reduced in the presence of interferon induced protein with tetratricopeptide repeats 3 (IFIT3). COPS5 contains a JAMM motif (aa 138-151) that is shown to be essential for the protease activity of the CSN complex. Higher levels of COPS5 are observed in a variety of human cancers and are correlated with a poor prognosis. (Ref.: Kato, J-Y., et al (2006). Hybridoma. 25(6); 342-348).

外觀

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at +2°C to +8°C from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable