MABC1102

Anti-DKK2 Antibody, clone 5F8

clone 5F8, from mouse

• 别名: Dickkopf-related protein 2, Dickkopf-2, hDkk-2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 5F8, monoclonal
• 物種活性: human
• 物種活性（以同源性預測）: monkey (based on 100% sequence homology)
• 包裝: antibody small pack of 25 μL
• 技術: ELISA: suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_055236.1
• UniProt登錄號: Q9UBU2
• 目標翻譯後修改: unmodified
• 基因資訊: human ... DKK2(27123)

说明

一般說明

Dickkopf-related protein 2 (UniProt: Q9UBU2; also known as Dickkopf-2, DKK2, Dkk-2, hDkk-2) is encoded by the DKK2 (also known as UNQ682/PRO1316) gene (Gene ID: 27123) in human. DKK2 is a member of the Dickkopf family that is expressed in heart, brain, skeletal muscle, and lung. It is synthesized with a signal peptide (aa 1-33) that is subsequently cleaved off in the mature form. DKK2 is reported to antagonize canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. Its C-terminal cysteine-rich domain mediates its interaction with LRP5 and LRP6. It plays play an important role in vertebrate development, where it locally inhibits Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. DKK2 secreted by tumor cells is shown to act on cytotoxic lymphocytes and inhibit STAT5 signaling by blocking STAT5 nuclear localization via LRP5, independent of LRP6. Loss of DKK2 is reported to activate natural killer (NK) cells and CD8+ T cells in tumors that can delay tumor progression and enhance the effects of PD-1 blockade. (Ref.: Xiao, Q., et al. (2018). Nat. Med. 24(3): 262-270).

特異性

Clone 5F8 specifically detects human Dickkopf-related protein 2. It targets an epitope with in 15 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Dickkopf-2 protein.

應用

Anti-DKK2, clone 5F8, Cat. No. MABC1102, is a mouse monoclonal antibody that detects Dickkopf-related protein 2 and has been tested for use in ELISA, Inhibits Activity/Function, and Western Blotting.

Inhibits Activity/Function Analysis: A representative lot inhibited DKK2-mediated, but not DKK1-mediated, antagonism of Wnt activation. (Xiao, Q., et. al. (2018). Nat Med. 24(3):262-270).


ELISA Analysis: A representative lot detected DKK2 in ELISA applications (Xiao, Q., et. al. (2018). Nat Med. 24(3):262-270).

Research Category
Apoptosis & Cancer

品質

Evaluated by Western Blotting in human heart tissue lysates.

Western Blotting Analysis: A 1:250 dilution of this antibody detected DKK2 in human heart tissue lysates.

標靶描述

~38 kDa observed; 28.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.