推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
19.4E, monoclonal
物種活性
monkey, human
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG1
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... MUC5B(727897)
一般說明
Mucins are a family of highly glycosylated proteins that are present on the surface of, and are secreted by, many glandular epithelial cells. Mucins provide a barrier between the luminal membranes of cells in the epithelium and their environment. For instance, in the digestive tract, the mucin barrier protects the epithelial cells from the action of proteolytic enzymes. These high molecular weight glycoproteins consist of a nonglobular protein backbone (apomucin) and many O-linked glycans. Salivary Mucin 5B proteins are some of the largest polypeptides, with molecular weights ranging from 14 - 40 x 103 kDa.
特異性
Reacts with Mucin 5B (MUC5B). Does not react with human Mucin 5A or 5C.
免疫原
Un-glycosylated, serine- and threonine-free synthetic peptide from the N-terminus of human MUC5B.
應用
Detect Mucin 5B using this Anti-Mucin 5B Antibody, clone 19.4E validated for use in ELISA, WB, IH.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Tumor Markers
Tumor Markers
Western blot; Bands in the high molecular weight range of 200kDa or above are expected for highly glycosylated proteins.
Immunohistchemistry: Paraffin sections. Deglycosylating the tissue is critical to expose epitope. There are a number of deglycosylation protocols available and a discussion of these related to Mucin can be found in Cao, Y. et al. (1997)(see references).
ELISA
Optimal working dilutions must be determined by end user.
Immunohistchemistry: Paraffin sections. Deglycosylating the tissue is critical to expose epitope. There are a number of deglycosylation protocols available and a discussion of these related to Mucin can be found in Cao, Y. et al. (1997)(see references).
ELISA
Optimal working dilutions must be determined by end user.
外觀
Format: Purified
Purified immunoglobulin. Liquid in PBS containing 0.1% sodium azide.
儲存和穩定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
D J Thornton et al.
Glycobiology, 9(3), 293-302 (1999-02-20)
The MG1 population of mucins was isolated from human whole salivas by gel chromatography followed by isopycnic density gradient centrifugation. The reduced and alkylated MG1 mucins, separated by anion exchange chromatography, were of similar size (radius of gyration 55-64 nm)
Kristina A Thomsson et al.
Glycobiology, 12(1), 1-14 (2002-02-05)
The high-molecular-mass salivary mucin MG1, one of two major mucins produced by human salivary glands, plays an important role in oral health by coating the tooth surface and by acting as a bacterial receptor. Here this mucin was purified from
Mucins (MUC1 and MUC3) of gastrointestinal and breast epithelia reveal different and heterogeneous tumor-associated aberrations in glycosylation
Cao, Y, et al
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45, 1547-1557 (1997)
MUC5B glycosylation in human saliva reflects blood group and secretor status
Thomsson, Kristina A, et al
Glycobiology, 15, 791-804 (2005)
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