推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
55K2, monoclonal
物種活性
echinoderm, sea urchin, gerbil, human
物種活性（以同源性預測）
mammals
製造商／商標名
Chemicon®
技術
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同型
IgG1
適合性
not suitable for immunoprecipitation
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... FSCN1(6624)
特異性
Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.
免疫原
Human fascin
應用
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.
Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.
Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.
Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.
Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.
Optimal working dilutions must be determined by the end user.
外觀
Format: Purified
Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
儲存和穩定性
Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.
分析報告
Control
POSITIVE CONTROL:
Hela cells, neuronal tissues, antigen presenting dendritic cells.
POSITIVE CONTROL:
Hela cells, neuronal tissues, antigen presenting dendritic cells.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
S Al-Saad et al.
British journal of cancer, 99(9), 1476-1483 (2008-10-16)
Vimentin, nuclear factor-kappaB (NF-kappaB) p105, fascin, E-cadherin, TGF-beta, Par6 and atypical PKC are molecular markers that play an important role in cell differentiation. Herein, we investigate their prognostic impact in primary non-small-cell carcinoma (NSCLC). Tumour tissue samples from 335 resected
The prognostic impact of TGF-?1, fascin, NF-?B and PKC-? expression in soft tissue sarcomas.
Valkov, A; Sorbye, SW; Kilvaer, TK; Donnem, T; Smeland, E; Bremnes, RM; Busund, LT
Testing null
Protein expression dynamics during postnatal mouse brain development.
Laeremans, A; Van de Plas, B; Clerens, S; Van den Bergh, G; Arckens, L; Hu, TT
Journal of experimental neuroscience null
C S Cohan et al.
Cell motility and the cytoskeleton, 48(2), 109-120 (2001-02-13)
Growth cones at the distal tips of growing nerve axons contain bundles of actin filaments distributed throughout the lamellipodium and that project into filopodia. The regulation of actin bundling by specific actin binding proteins is likely to play an important
S Yamashiro-Matsumura et al.
The Journal of cell biology, 103(2), 631-640 (1986-08-01)
The 55-kD protein is a new actin-bundling protein purified from HeLa cells (Yamashiro-Matsumura, S., and F. Matsumura, 1985, J. Biol. Chem., 260:5087-5097). We have prepared monoclonal antibodies against the 55-kD protein and examined its intracellular localization, as well as its
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