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Key Documents

MAB3224

Sigma-Aldrich

Anti-Basal Cell Cytokeratin Antibody

Chemicon®, from mouse

别名:

Cytokeratin 5 and others

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.43

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

monoclonal

物種活性

rat, quail, guinea pig, human, hamster, canine, rabbit, pig, chicken

製造商/商標名

Chemicon®

技術

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

同型

IgG1

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... KRT5(3852)

一般說明

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.

特異性

MAB3224 is a broadly reacting cytokeratin antibody which reacts with cytokeratin 5 among others. It stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cell in the prostate epithelium.

免疫原

Human cytokeratins.

應用

Anti-Basal Cell Cytokeratin Antibody detects level of Basal Cell Cytokeratin & has been published & validated for use in FC, IC, IH & WB.
Research Category
Cell Structure
Research Sub Category
Cytokeratins
Western blot: 1:100-1:1,000
Immunohistochemistry on frozen tissue sections: 1:100-1:200
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200
Optimal working dilutions must be determined by the end user.

外觀

Format: Purified
Purified immunoglobulin. Purified immunoglobulin in buffer with 0.1% sodium azide.

儲存和穩定性

Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Development of a novel monoclonal antibody recognizing basal cells of human squamous epithelia.
Jiro Hitomi, Fumio Ishizaki, Eiji Kimura, Nobuyuki Sato
Archives of Histology and Cytology null
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Manuela Saathoff, Barbara Blum, Thomas Quast, Gregor Kirfel, Volker Herzog
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Oct4A is expressed by a subpopulation of prostate neuroendocrine cells.
Sotomayor, P; Godoy, A; Smith, GJ; Huss, WJ
Prostate null

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