生物源
mouse
品質等級
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
R4, monoclonal
物種活性
guinea pig, bovine, mouse, rat, human
製造商／商標名
Chemicon®
技術
western blot: suitable
同型
IgG2b
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
bovine ... Gnai1(281790)
human ... GNAI1(2770)
mouse ... Gnai1(14677)
rat ... Gnai1(25686)
特異性
Gialpha-1. Strong reactivity with rat (100%), mouse (78%), bovine (74%) and guinea pig (48%). Slight reactivity with human (31%). Specificity confirmed by Western blot versus bovine Galphat, rat Goalpha, rat Gialpha-1, Gialpha-2, rat Gialpha-3, bovine Gsalpha, and murine GoalphaA and GoalphaB.
免疫原
Rat brain G-proteins purified by the method of Sternweis and Robishaw (Sternweis & Robishaw, 1984).
應用
Anti-G Protein Giα-1 Antibody, clone R4 detects level of G Protein Giα-1 & has been published & validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Western blot 1:500 - 1:2000.
Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.
Optimal working dilutions must be determined by end user.
Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.
Optimal working dilutions must be determined by end user.
外觀
Liquid ascites, containing sodium azide as a preservative.
儲存和穩定性
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain.
Sternweis, P C and Robishaw, J D
The Journal of Biological Chemistry, 259, 13806-13813 (1984)
E Burgos-Ramos et al.
Neuroscience, 154(4), 1458-1466 (2008-06-17)
Minocycline is a semi-synthetic second-generation tetracycline known to improve cognition in amyloid precursor protein transgenic mice. Whether it can protect the somatostatin (SRIF) receptor-effector system, also involved in learning and memory, from alterations induced by chronic i.c.v. infusion of beta-amyloid
Activity of the hippocampal somatostatinergic system following daily administration of melatonin.
Rosa Maria Izquierdo-Claros, Maria Del Carmen Boyano-Adanez, Eduardo Arilla-Ferreiro
Brain Research Molecular Brain Research null
Acutely administered melatonin decreases somatostatin-binding sites and the inhibitory effect of somatostatin on adenylyl cyclase activity in the rat hippocampus.
Rosa Maria Izquierdo-Claros, Maria del Carmen Boyano-Adanez Md, Eduardo Arilla-Ferreiro
Journal of Pineal Research null
Apurva A Javkhedkar et al.
Physiological reports, 3(11) (2015-11-26)
Previously we have shown that in spontaneously hypertensive rats (SHR) renal angiotensin (Ang) II receptor (AT1R) upregulation leads to overstimulation of Na/K-ATPase by Ang II. There are reports that antioxidants can reduce oxidative stress and blood pressure (BP) in SHR
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