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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
3A3, monoclonal
物種活性
human
物種活性(以同源性預測)
bovine (based on 100% sequence homology), rat (98% sequence homology), mouse (98% sequence homology)
技術
immunocytochemistry: suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... FERMT2(10979)
一般說明
Kindlin家族是一组黏着斑蛋白,由于它们具有结合和激活整联蛋白的能力而成为人们关注的焦点。这种能力的异常已将Kindlin与几种遗传和获得性疾病联系起来,例如Kindler综合征,癌症和白细胞粘附缺乏症。亚型Kindlin-2专门参与将migfilin蛋白募集到局部粘连位点以进行细胞ECM各种相互作用,协调肌动蛋白的生产和细胞形状的修饰。它还积极参与连接ECM粘连位点和肌动蛋白细胞骨架(Shi,2007)。Kindlin-2在整个组织中普遍表达。
特異性
该抗体识别Kindlin-2。
免疫原
GST 标记的重组蛋白对应于人Kindlin-2。
表位:未知
應用
研究子类别
细胞骨架
细胞骨架
研究类别
细胞结构
细胞结构
该抗Kindlin-2抗体克隆3A3经过验证可用在 WB,IC 中检测Kindlin-2。
品質
通过蛋白质印迹在HeLa 细胞裂解物中进行了评估。
蛋白质印迹分析:0.5 µg/ml该抗体可在10 µg HeLa细胞裂解物中检测到Kindlin-2。
蛋白质印迹分析:0.5 µg/ml该抗体可在10 µg HeLa细胞裂解物中检测到Kindlin-2。
標靶描述
~70 kDa
外觀
在含0.1 M Tris-甘氨酸(pH 7.4,150 mM NaCl)和0.05%叠氮化钠的缓冲液中的纯化的小鼠单克隆IgG1κ。
形式:纯化
纯化的蛋白G
儲存和穩定性
自收到之日起,在 2-8°C 条件下可稳定保存1年
分析報告
对照
HeLa细胞裂解物
HeLa细胞裂解物
其他說明
浓度:请参考批次特异性浓缩物的分析证书。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Journal of experimental & clinical cancer research : CR, 36(1), 134-134 (2017-10-04)
Kindlin-2 is a member of the focal adhesion protein family that regulates invasion and metastasis in multiple malignancies; however, little is known about the role of Kindlin-2 in hepatocellular carcinoma (HCC) progression. Immunohistochemistry was used to investigate Kindlin-2 expression in
Cell death & disease, 10(12), 890-890 (2019-11-27)
Kindlin-2 plays an important role in the regulation of cardiac structure and function. Depletion of Kindlin-2 contributes to cardiac hypertrophy and progressive heart failure, however, the precise mechanisms involved in this process remain unclear. GATA4 is a critical transcription factor
JCI insight, 4(13) (2019-07-12)
Kindlin-2 regulates integrin-mediated cell adhesion to and migration on the extracellular matrix. Our recent studies demonstrate important roles of kindlin-2 in regulation of mesenchymal stem cell differentiation and skeletal development. In this study, we generated adipose tissue-specific conditional knockout of
FEBS letters, 589(16), 2155-2162 (2015-07-06)
Kindlin-2, as an integrin-interacting protein, was known to be required for the maintenance of cardiac structure and function in zebrafish. However, the mechanism remains unclear. We found that Kindlin-2 interacts and colocalizes with α-actinin-2 at the Z-disc of mouse cardiac
Nature communications, 13(1), 2362-2362 (2022-04-30)
Talin-induced integrin binding to extracellular matrix ligands (integrin activation) is the key step to trigger many fundamental cellular processes including cell adhesion, cell migration, and spreading. Talin is widely known to use its N-terminal head domain (talin-H) to bind and
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