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Key Documents

MAB2137

Sigma-Aldrich

Anti-Homing Receptor Antibody, clone A020

clone A020, Chemicon®, from rat

别名:

CD44s, HCAM, Pgp-1

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rat

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

A020, monoclonal

物種活性

mouse, human, rat, pig, rabbit, canine, bovine

製造商/商標名

Chemicon®

技術

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

同型

IgG2b

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... CD44(960)

特異性

Antibody reacts with the standard CD44 isoform (CD44s) that lacks exon sequences v1-v10. Also called human CD44 (85-95 kDa) gp90{Hermes} and mouse gp85 (Pgp-1).

免疫原

Purified human blood lymphocyte CD44

應用

Anti-Homing Receptor Antibody, clone A020 is an antibody against Homing Receptor for use in ELISA, FC, IC, IH & WB.
ELISA 1:2000

Flow Cytometry :1:1000

Immunofluorescence 1:200

Immunohistostaining of paraffin embedded tissues. 1:250; antigen retrieval is usually not needed.

Immunoprecipitation 1:200

Western blotting: non-reduced conditions only; 90kDa band is detected; blocking PBS/5% milk protein, primary antibody dilution 1:1000 o/n incubation.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

標靶描述

90 kDa

外觀

Protein A purified
Format: Purified
Liquid in 10 mM sodium phosphate, pH 7.5 with 150 mM NaCl and 0.01% sodium azide.

儲存和穩定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析報告

Control
Cells of epithelium and highly expressed by carcinomas

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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M Z Gilcrease et al.
Cancer, 86(11), 2320-2326 (1999-12-11)
Recent reports have shown altered expression of CD44 in renal cell carcinomas. However, to the authors' knowledge there are no data correlating CD44 expression in renal cell carcinomas with subsequent tumor progression or recurrence, nor is there information about the
Milan Makwana et al.
The Journal of comparative neurology, 518(5), 699-721 (2009-12-25)
Generation of new axonal sprouts plays an important role in neural repair. In the current study, we examined the appearance, composition and effects of gene deletions on intrabrainstem sprouts following peripheral facial nerve axotomy. Axotomy was followed by the appearance
Mature human thymocytes migrate on laminin-5 with activation of metalloproteinase-14 and cleavage of CD44.
Vivinus-Nebot, Mylene, et al.
Journal of immunology (Baltimore, Md. : 1950), 172, 1397-1406 (2004)
Sameerah Shaheen et al.
Stem cell reviews and reports, 12(4), 492-499 (2016-05-22)
Colorectal cancers (CRCs) form a disorganized hierarchy of heterogeneous cell populations on which current chemotherapy regimens fail to exert their distinctive cytotoxicity. A small sub-population of poorly differentiated cancer stem-like cells (CSCs), also known as cancer initiating cells, may exhibit
Carmela Ricciardelli et al.
BMC cancer, 13, 476-476 (2013-10-16)
Hyaluronan (HA) an important component of the extracellular matrix, has been linked to tumor progression and drug resistance in several malignancies. However, limited data is available for ovarian cancer. This study investigated the role of hyaluronan (HA) and a potential

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