MAB1998Z
Anti-Integrin α2β1 Antibody, clone BHA2.1, azide free
clone BHA2.1, Chemicon®, from mouse
别名:
VLA-2, MAB1998
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所有图片(2)
About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
無性繁殖:
BHA2.1, monoclonal
application:
FACS
IHC
IP
IHC
IP
物種活性:
human, pig
技術:
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
citations:
9
推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
BHA2.1, monoclonal
物種活性
human, pig
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... ITGA2(3673)
特異性
Reacts with the collagen receptor VLA-2 (alpha2beta1) integrin complex.
應用
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin α2β1 Antibody, clone BHA2.1, azide free is validated for use in FC, IH, IP, FUNC for the detection of Integrin α2β1.
flow cytometry: 10 μg/mL
Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1
immunohistochemistry in frozen tissue sections: 5 μg/mL
Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.
Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.
Final working dilutions must be determined by end user.
Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1
immunohistochemistry in frozen tissue sections: 5 μg/mL
Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.
Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.
Final working dilutions must be determined by end user.
外觀
Protein A purified
Format: Purified
Purified immunoglobulin from Protein A chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing no preservative.
儲存和穩定性
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析報告
Control
Skin, CD4 & CD8 positive T cells
Skin, CD4 & CD8 positive T cells
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Christopher F Cummings et al.
The Journal of cell biology, 213(4), 479-494 (2016-05-25)
Basement membranes are defining features of the cellular microenvironment; however, little is known regarding their assembly outside cells. We report that extracellular Cl(-) ions signal the assembly of collagen IV networks outside cells by triggering a conformational switch within collagen
Platelet alpha2beta1 integrin activation: contribution of ligand internalization and the alpha2-cytoplasmic domain.
Wang, Zhengyan, et al.
Blood, 102, 1307-1315 (2003)
Integrin VLA-2 (alpha2beta1) function in postextravasation movement of human rhabdomyosarcoma RD cells in the liver
Hangan, D, et al
Cancer Research, 56, 3142-3149 (1996)
Seiji Goda et al.
The American journal of pathology, 169(2), 445-458 (2006-08-01)
Natural killer (NK) cells play a key role in inflammation and tumor regression through their ability to migrate into tissues. CXCL12 is a chemokine that promotes lymphocyte invasion and migration into tissues; however, the mechanism for this process remains incompletely
Josh Mauney et al.
Matrix biology : journal of the International Society for Matrix Biology, 28(5), 251-262 (2009-04-21)
Previously, we reported that the conformational transition of collagen I matrix plays, along with differentiation stimuli, a regulatory role in determination of differentiation lineage of bone marrow stromal sells via distinct signaling pathways specific for the structural state of the
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