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Key Documents

MAB1613

Sigma-Aldrich

抗驱动蛋白抗体(重链,非神经元KHC,克隆H1)

clone H1, Chemicon®, from mouse

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

H1, monoclonal

物種活性

rat, human, bovine

製造商/商標名

Chemicon®

技術

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

同型

IgG1

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... KIF5B(3799)

特異性

与非神经元 KHC的重链N末端附近的的主要形式反应。

免疫原

表位:重链,非神经元KHC

應用

在多聚甲醛固定的组织上进行免疫组化。也适用于EM。

免疫细胞化学:多聚甲醛固定的细胞; 5-10′ RT,新鲜制备的4% PFA;以 0.1-.2% triton X-100透化的3% 正常血清封闭缓冲液

免疫印迹:识别非神经元样品的 124kDa KHC

免疫沉淀

驱动蛋白介导的转运的抑制

EIA/RIA

最佳工作稀释度必须由最终用户进行确定。
抗驱动蛋白抗体(重链,非神经元KHC,克隆H1)可检测驱动蛋白水平 & 已有相关文献发表 & 经验证可用于ELISA、IP、RIA、WB、IC、IH。

外觀

形式:纯化
液体形式,溶于0.02 M磷酸盐缓冲液、0.25 M NaCl(pH 7.6)和0.1%叠氮化钠中。

其他說明

浓度:请参考批次特异性浓缩物的检验报告。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic.
Lippincott-Schwartz, J, et al.
The Journal of cell biology, 128, 293-306 (1995)
G Vancoillie et al.
The Journal of investigative dermatology, 114(3), 421-429 (2000-02-26)
Microtubuli play an important role in the organization of organelles and membrane traffic. They are present in melanocytic dendrites through which melanosomes are transported towards keratinocytes. Besides the actin-based motility systems, microtubuli-associated motor proteins also play a critical role in
Wen-Jun Wang et al.
Drug metabolism and disposition: the biological fate of chemicals, 42(1), 62-69 (2013-10-12)
Previous studies identified a family of organic anion transport proteins (OATPs), many of which have C-terminal PDZ binding consensus sequences. In particular, the C-terminal four amino acids of Oatp1a1, a transporter on rat and mouse hepatocytes, comprise a consensus binding
Hongtao Chen et al.
Biophysical journal, 94(4), 1508-1520 (2007-11-06)
The role of cholesterol in the regulation of endosome motility was investigated by monitoring the intracellular trafficking of endocytosed folate receptors (FRs) labeled with fluorescent folate conjugates. Real-time fluorescence imaging of HeLa cells transfected with green fluorescent protein-tubulin revealed that
Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration.
Hirokawa, N, et al.
Cell, 56, 867-878 (1989)

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